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Determination of Natamycin in Turkish Yoghurt
This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868916/ https://www.ncbi.nlm.nih.gov/pubmed/27274731 http://dx.doi.org/10.1155/2016/8480963 |
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author | Bilgic Alkaya, Dilek Karalomlu, Ozlem |
author_facet | Bilgic Alkaya, Dilek Karalomlu, Ozlem |
author_sort | Bilgic Alkaya, Dilek |
collection | PubMed |
description | This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented. |
format | Online Article Text |
id | pubmed-4868916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48689162016-06-05 Determination of Natamycin in Turkish Yoghurt Bilgic Alkaya, Dilek Karalomlu, Ozlem Int J Anal Chem Research Article This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented. Hindawi Publishing Corporation 2016 2016-05-03 /pmc/articles/PMC4868916/ /pubmed/27274731 http://dx.doi.org/10.1155/2016/8480963 Text en Copyright © 2016 D. Bilgic Alkaya and O. Karalomlu. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bilgic Alkaya, Dilek Karalomlu, Ozlem Determination of Natamycin in Turkish Yoghurt |
title | Determination of Natamycin in Turkish Yoghurt |
title_full | Determination of Natamycin in Turkish Yoghurt |
title_fullStr | Determination of Natamycin in Turkish Yoghurt |
title_full_unstemmed | Determination of Natamycin in Turkish Yoghurt |
title_short | Determination of Natamycin in Turkish Yoghurt |
title_sort | determination of natamycin in turkish yoghurt |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868916/ https://www.ncbi.nlm.nih.gov/pubmed/27274731 http://dx.doi.org/10.1155/2016/8480963 |
work_keys_str_mv | AT bilgicalkayadilek determinationofnatamycininturkishyoghurt AT karalomluozlem determinationofnatamycininturkishyoghurt |