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BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma

BACKGROUND: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. OBJECTIVE: The goal of this research was to determine the prevalence of the BRAF V...

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Autores principales: Zepeda-Lopez, Priscilla Denise, Salas-Alanis, Julio Cesar, Toussaint-Caire, Sonia, Gutierrez-Mendoza, Daniela, Vega-Memije, Elisa, Silva, Saúl Lino, Fajardo-Ramírez, Oscar Raul, Alcazar, Gregorio, Moreno-Treviño, María Guadalupe, Saldaña, Hugo Alberto Barrera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: S. Karger AG 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868925/
https://www.ncbi.nlm.nih.gov/pubmed/27194985
http://dx.doi.org/10.1159/000445939
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author Zepeda-Lopez, Priscilla Denise
Salas-Alanis, Julio Cesar
Toussaint-Caire, Sonia
Gutierrez-Mendoza, Daniela
Vega-Memije, Elisa
Silva, Saúl Lino
Fajardo-Ramírez, Oscar Raul
Alcazar, Gregorio
Moreno-Treviño, María Guadalupe
Saldaña, Hugo Alberto Barrera
author_facet Zepeda-Lopez, Priscilla Denise
Salas-Alanis, Julio Cesar
Toussaint-Caire, Sonia
Gutierrez-Mendoza, Daniela
Vega-Memije, Elisa
Silva, Saúl Lino
Fajardo-Ramírez, Oscar Raul
Alcazar, Gregorio
Moreno-Treviño, María Guadalupe
Saldaña, Hugo Alberto Barrera
author_sort Zepeda-Lopez, Priscilla Denise
collection PubMed
description BACKGROUND: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. OBJECTIVE: The goal of this research was to determine the prevalence of the BRAF V600E mutation in patients from Central Mexico diagnosed with primary melanoma. METHODS: Skin biopsies from 47 patients with melanoma were obtained from the dermatology department of the Hospital General ‘Dr. Manuel Gea González’ in Mexico City. For BRAF mutation determination, after DNA isolation, the gene region where the mutation occurs was amplified by PCR. Subsequently, the presence or absence of the V600E mutation was detected by Sanger sequencing performed at the private molecular diagnostic laboratory Vitagénesis in Monterrey, Mexico. RESULTS: Of the 47 patients sampled, 6.4% harbored the V600E mutation. No statistical significance was found between mutations and the type of tumor.
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spelling pubmed-48689252016-05-18 BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma Zepeda-Lopez, Priscilla Denise Salas-Alanis, Julio Cesar Toussaint-Caire, Sonia Gutierrez-Mendoza, Daniela Vega-Memije, Elisa Silva, Saúl Lino Fajardo-Ramírez, Oscar Raul Alcazar, Gregorio Moreno-Treviño, María Guadalupe Saldaña, Hugo Alberto Barrera Case Rep Oncol Published online: April, 2016 BACKGROUND: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. OBJECTIVE: The goal of this research was to determine the prevalence of the BRAF V600E mutation in patients from Central Mexico diagnosed with primary melanoma. METHODS: Skin biopsies from 47 patients with melanoma were obtained from the dermatology department of the Hospital General ‘Dr. Manuel Gea González’ in Mexico City. For BRAF mutation determination, after DNA isolation, the gene region where the mutation occurs was amplified by PCR. Subsequently, the presence or absence of the V600E mutation was detected by Sanger sequencing performed at the private molecular diagnostic laboratory Vitagénesis in Monterrey, Mexico. RESULTS: Of the 47 patients sampled, 6.4% harbored the V600E mutation. No statistical significance was found between mutations and the type of tumor. S. Karger AG 2016-04-21 /pmc/articles/PMC4868925/ /pubmed/27194985 http://dx.doi.org/10.1159/000445939 Text en Copyright © 2016 by S. Karger AG, Basel http://creativecommons.org/licenses/by-nc/4.0/ This article is licensed under the Creative Commons Attribution-NonCommercial-4.0 International License (CC BY-NC) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes requires written permission.
spellingShingle Published online: April, 2016
Zepeda-Lopez, Priscilla Denise
Salas-Alanis, Julio Cesar
Toussaint-Caire, Sonia
Gutierrez-Mendoza, Daniela
Vega-Memije, Elisa
Silva, Saúl Lino
Fajardo-Ramírez, Oscar Raul
Alcazar, Gregorio
Moreno-Treviño, María Guadalupe
Saldaña, Hugo Alberto Barrera
BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
title BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
title_full BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
title_fullStr BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
title_full_unstemmed BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
title_short BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
title_sort braf mutation (v600e) prevalence in mexican patients diagnosed with melanoma
topic Published online: April, 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868925/
https://www.ncbi.nlm.nih.gov/pubmed/27194985
http://dx.doi.org/10.1159/000445939
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