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Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage

This study aimed to inhibit Escherichia coli (E. coli) O157:H7 artificially contaminated in fresh meat using bacteriophage. Among 14 bacteriophages, the highly lytic bacteriophage BPECO19 strain was selected to inhibit E. coli O157:H7 in artificially contaminated meat samples. Bacteriophage BPECO19...

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Autores principales: Seo, Jina, Seo, Dong Joo, Oh, Hyejin, Jeon, Su Been, Oh, Mi-Hwa, Choi, Changsun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4869544/
https://www.ncbi.nlm.nih.gov/pubmed/27194926
http://dx.doi.org/10.5851/kosfa.2016.36.2.186
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author Seo, Jina
Seo, Dong Joo
Oh, Hyejin
Jeon, Su Been
Oh, Mi-Hwa
Choi, Changsun
author_facet Seo, Jina
Seo, Dong Joo
Oh, Hyejin
Jeon, Su Been
Oh, Mi-Hwa
Choi, Changsun
author_sort Seo, Jina
collection PubMed
description This study aimed to inhibit Escherichia coli (E. coli) O157:H7 artificially contaminated in fresh meat using bacteriophage. Among 14 bacteriophages, the highly lytic bacteriophage BPECO19 strain was selected to inhibit E. coli O157:H7 in artificially contaminated meat samples. Bacteriophage BPECO19 significantly reduced E. coli O157:H7 bacterial load in vitro in a multiplicity of infection (MOI)-dependent manner. E. coli O157:H7 was completely inhibited only in 10 min in vitro by the treatment of 10,000 MOI BPECO19. The treatment of BPECO19 at 100,000 MOI completely reduced 5 Log CFU/cm(2) E. coli O157:H7 bacterial load in beef and pork at 4 and 8h, respectively. In chicken meat, a 4.65 log reduction of E. coli O157:H7 was observed at 4 h by 100,000 MOI. The treatment of single bacteriophage BPECO19 was an effective method to control E. coli O157:H7 in meat samples.
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spelling pubmed-48695442016-05-18 Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage Seo, Jina Seo, Dong Joo Oh, Hyejin Jeon, Su Been Oh, Mi-Hwa Choi, Changsun Korean J Food Sci Anim Resour Article This study aimed to inhibit Escherichia coli (E. coli) O157:H7 artificially contaminated in fresh meat using bacteriophage. Among 14 bacteriophages, the highly lytic bacteriophage BPECO19 strain was selected to inhibit E. coli O157:H7 in artificially contaminated meat samples. Bacteriophage BPECO19 significantly reduced E. coli O157:H7 bacterial load in vitro in a multiplicity of infection (MOI)-dependent manner. E. coli O157:H7 was completely inhibited only in 10 min in vitro by the treatment of 10,000 MOI BPECO19. The treatment of BPECO19 at 100,000 MOI completely reduced 5 Log CFU/cm(2) E. coli O157:H7 bacterial load in beef and pork at 4 and 8h, respectively. In chicken meat, a 4.65 log reduction of E. coli O157:H7 was observed at 4 h by 100,000 MOI. The treatment of single bacteriophage BPECO19 was an effective method to control E. coli O157:H7 in meat samples. Korean Society for Food Science of Animal Resources 2016 2016-04-30 /pmc/articles/PMC4869544/ /pubmed/27194926 http://dx.doi.org/10.5851/kosfa.2016.36.2.186 Text en Copyright © 2016, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Seo, Jina
Seo, Dong Joo
Oh, Hyejin
Jeon, Su Been
Oh, Mi-Hwa
Choi, Changsun
Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage
title Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage
title_full Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage
title_fullStr Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage
title_full_unstemmed Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage
title_short Inhibiting the Growth of Escherichia coli O157:H7 in Beef, Pork, and Chicken Meat using a Bacteriophage
title_sort inhibiting the growth of escherichia coli o157:h7 in beef, pork, and chicken meat using a bacteriophage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4869544/
https://www.ncbi.nlm.nih.gov/pubmed/27194926
http://dx.doi.org/10.5851/kosfa.2016.36.2.186
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