Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas

Loss of functional retinal ganglion cells (RGC) is an element of retinal degeneration that is poorly understood. This is in part due to the lack of a reliable and validated protocol for the isolation of primary RGCs. Here we optimize a feasible, reproducible, standardized flow cytometry-based protoc...

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Detalles Bibliográficos
Autores principales: Chintalapudi, Sumana R., Djenderedjian, Levon, Stiemke, Andrew B., Steinle, Jena J., Jablonski, Monica M., Morales-Tirado, Vanessa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870266/
https://www.ncbi.nlm.nih.gov/pubmed/27242509
http://dx.doi.org/10.3389/fnagi.2016.00093

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