Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets

We demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resoluti...

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Detalles Bibliográficos
Autores principales: Zhao, Teng, Lau, Sze Cheung, Wang, Ying, Su, Yumian, Wang, Hao, Cheng, Aifang, Herrup, Karl, Ip, Nancy Y., Du, Shengwang, Loy, M. M. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870613/
https://www.ncbi.nlm.nih.gov/pubmed/27189786
http://dx.doi.org/10.1038/srep26159
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author Zhao, Teng
Lau, Sze Cheung
Wang, Ying
Su, Yumian
Wang, Hao
Cheng, Aifang
Herrup, Karl
Ip, Nancy Y.
Du, Shengwang
Loy, M. M. T.
author_facet Zhao, Teng
Lau, Sze Cheung
Wang, Ying
Su, Yumian
Wang, Hao
Cheng, Aifang
Herrup, Karl
Ip, Nancy Y.
Du, Shengwang
Loy, M. M. T.
author_sort Zhao, Teng
collection PubMed
description We demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution.
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spelling pubmed-48706132016-06-01 Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets Zhao, Teng Lau, Sze Cheung Wang, Ying Su, Yumian Wang, Hao Cheng, Aifang Herrup, Karl Ip, Nancy Y. Du, Shengwang Loy, M. M. T. Sci Rep Article We demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution. Nature Publishing Group 2016-05-18 /pmc/articles/PMC4870613/ /pubmed/27189786 http://dx.doi.org/10.1038/srep26159 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhao, Teng
Lau, Sze Cheung
Wang, Ying
Su, Yumian
Wang, Hao
Cheng, Aifang
Herrup, Karl
Ip, Nancy Y.
Du, Shengwang
Loy, M. M. T.
Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
title Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
title_full Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
title_fullStr Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
title_full_unstemmed Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
title_short Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
title_sort multicolor 4d fluorescence microscopy using ultrathin bessel light sheets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870613/
https://www.ncbi.nlm.nih.gov/pubmed/27189786
http://dx.doi.org/10.1038/srep26159
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