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Infused polymers for cell sheet release

Tissue engineering using whole, intact cell sheets has shown promise in many cell-based therapies. However, current systems for the growth and release of these sheets can be expensive to purchase or difficult to fabricate, hindering their widespread use. Here, we describe a new approach to cell shee...

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Autores principales: Juthani, Nidhi, Howell, Caitlin, Ledoux, Haylea, Sotiri, Irini, Kelso, Susan, Kovalenko, Yevgen, Tajik, Amanda, Vu, Thy L., Lin, Jennifer J., Sutton, Amy, Aizenberg, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870626/
https://www.ncbi.nlm.nih.gov/pubmed/27189419
http://dx.doi.org/10.1038/srep26109
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author Juthani, Nidhi
Howell, Caitlin
Ledoux, Haylea
Sotiri, Irini
Kelso, Susan
Kovalenko, Yevgen
Tajik, Amanda
Vu, Thy L.
Lin, Jennifer J.
Sutton, Amy
Aizenberg, Joanna
author_facet Juthani, Nidhi
Howell, Caitlin
Ledoux, Haylea
Sotiri, Irini
Kelso, Susan
Kovalenko, Yevgen
Tajik, Amanda
Vu, Thy L.
Lin, Jennifer J.
Sutton, Amy
Aizenberg, Joanna
author_sort Juthani, Nidhi
collection PubMed
description Tissue engineering using whole, intact cell sheets has shown promise in many cell-based therapies. However, current systems for the growth and release of these sheets can be expensive to purchase or difficult to fabricate, hindering their widespread use. Here, we describe a new approach to cell sheet release surfaces based on silicone oil-infused polydimethylsiloxane. By coating the surfaces with a layer of fibronectin (FN), we were able to grow mesenchymal stem cells to densities comparable to those of tissue culture polystyrene controls (TCPS). Simple introduction of oil underneath an edge of the sheet caused it to separate from the substrate. Characterization of sheets post-transfer showed that they retain their FN layer and morphology, remain highly viable, and are able to grow and proliferate normally after transfer. We expect that this method of cell sheet growth and detachment may be useful for low-cost, flexible, and customizable production of cellular layers for tissue engineering.
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spelling pubmed-48706262016-06-01 Infused polymers for cell sheet release Juthani, Nidhi Howell, Caitlin Ledoux, Haylea Sotiri, Irini Kelso, Susan Kovalenko, Yevgen Tajik, Amanda Vu, Thy L. Lin, Jennifer J. Sutton, Amy Aizenberg, Joanna Sci Rep Article Tissue engineering using whole, intact cell sheets has shown promise in many cell-based therapies. However, current systems for the growth and release of these sheets can be expensive to purchase or difficult to fabricate, hindering their widespread use. Here, we describe a new approach to cell sheet release surfaces based on silicone oil-infused polydimethylsiloxane. By coating the surfaces with a layer of fibronectin (FN), we were able to grow mesenchymal stem cells to densities comparable to those of tissue culture polystyrene controls (TCPS). Simple introduction of oil underneath an edge of the sheet caused it to separate from the substrate. Characterization of sheets post-transfer showed that they retain their FN layer and morphology, remain highly viable, and are able to grow and proliferate normally after transfer. We expect that this method of cell sheet growth and detachment may be useful for low-cost, flexible, and customizable production of cellular layers for tissue engineering. Nature Publishing Group 2016-05-18 /pmc/articles/PMC4870626/ /pubmed/27189419 http://dx.doi.org/10.1038/srep26109 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Juthani, Nidhi
Howell, Caitlin
Ledoux, Haylea
Sotiri, Irini
Kelso, Susan
Kovalenko, Yevgen
Tajik, Amanda
Vu, Thy L.
Lin, Jennifer J.
Sutton, Amy
Aizenberg, Joanna
Infused polymers for cell sheet release
title Infused polymers for cell sheet release
title_full Infused polymers for cell sheet release
title_fullStr Infused polymers for cell sheet release
title_full_unstemmed Infused polymers for cell sheet release
title_short Infused polymers for cell sheet release
title_sort infused polymers for cell sheet release
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870626/
https://www.ncbi.nlm.nih.gov/pubmed/27189419
http://dx.doi.org/10.1038/srep26109
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