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Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes

Toxoplasma gondii infection induces alteration of the host cell cycle and cell proliferation. These changes are not only seen in directly invaded host cells but also in neighboring cells. We tried to identify whether this alteration can be mediated by exosomes secreted by T. gondii-infected host cel...

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Autores principales: Kim, Min Jae, Jung, Bong-Kwang, Cho, Jaeeun, Song, Hyemi, Pyo, Kyung-Ho, Lee, Ji Min, Kim, Min-Kyung, Chai, Jong-Yil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870968/
https://www.ncbi.nlm.nih.gov/pubmed/27180572
http://dx.doi.org/10.3347/kjp.2016.54.2.147
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author Kim, Min Jae
Jung, Bong-Kwang
Cho, Jaeeun
Song, Hyemi
Pyo, Kyung-Ho
Lee, Ji Min
Kim, Min-Kyung
Chai, Jong-Yil
author_facet Kim, Min Jae
Jung, Bong-Kwang
Cho, Jaeeun
Song, Hyemi
Pyo, Kyung-Ho
Lee, Ji Min
Kim, Min-Kyung
Chai, Jong-Yil
author_sort Kim, Min Jae
collection PubMed
description Toxoplasma gondii infection induces alteration of the host cell cycle and cell proliferation. These changes are not only seen in directly invaded host cells but also in neighboring cells. We tried to identify whether this alteration can be mediated by exosomes secreted by T. gondii-infected host cells. L6 cells, a rat myoblast cell line, and RH strain of T. gondii were selected for this study. L6 cells were infected with or without T. gondii to isolate exosomes. The cellular growth patterns were identified by cell counting with trypan blue under confocal microscopy, and cell cycle changes were investigated by flow cytometry. L6 cells infected with T. gondii showed decreased proliferation compared to uninfected L6 cells and revealed a tendency to stay at S or G2/M cell phase. The treatment of exosomes isolated from T. gondii-infected cells showed attenuation of cell proliferation and slight enhancement of S phase in L6 cells. The cell cycle alteration was not as obvious as reduction of the cell proliferation by the exosome treatment. These changes were transient and disappeared at 48 hr after the exosome treatment. Microarray analysis and web-based tools indicated that various exosomal miRNAs were crucial for the regulation of target genes related to cell proliferation. Collectively, our study demonstrated that the exosomes originating from T. gondii could change the host cell proliferation and alter the host cell cycle.
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spelling pubmed-48709682016-05-18 Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes Kim, Min Jae Jung, Bong-Kwang Cho, Jaeeun Song, Hyemi Pyo, Kyung-Ho Lee, Ji Min Kim, Min-Kyung Chai, Jong-Yil Korean J Parasitol Original Article Toxoplasma gondii infection induces alteration of the host cell cycle and cell proliferation. These changes are not only seen in directly invaded host cells but also in neighboring cells. We tried to identify whether this alteration can be mediated by exosomes secreted by T. gondii-infected host cells. L6 cells, a rat myoblast cell line, and RH strain of T. gondii were selected for this study. L6 cells were infected with or without T. gondii to isolate exosomes. The cellular growth patterns were identified by cell counting with trypan blue under confocal microscopy, and cell cycle changes were investigated by flow cytometry. L6 cells infected with T. gondii showed decreased proliferation compared to uninfected L6 cells and revealed a tendency to stay at S or G2/M cell phase. The treatment of exosomes isolated from T. gondii-infected cells showed attenuation of cell proliferation and slight enhancement of S phase in L6 cells. The cell cycle alteration was not as obvious as reduction of the cell proliferation by the exosome treatment. These changes were transient and disappeared at 48 hr after the exosome treatment. Microarray analysis and web-based tools indicated that various exosomal miRNAs were crucial for the regulation of target genes related to cell proliferation. Collectively, our study demonstrated that the exosomes originating from T. gondii could change the host cell proliferation and alter the host cell cycle. The Korean Society for Parasitology and Tropical Medicine 2016-04 2016-04-30 /pmc/articles/PMC4870968/ /pubmed/27180572 http://dx.doi.org/10.3347/kjp.2016.54.2.147 Text en © 2016, Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Min Jae
Jung, Bong-Kwang
Cho, Jaeeun
Song, Hyemi
Pyo, Kyung-Ho
Lee, Ji Min
Kim, Min-Kyung
Chai, Jong-Yil
Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
title Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
title_full Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
title_fullStr Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
title_full_unstemmed Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
title_short Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
title_sort exosomes secreted by toxoplasma gondii-infected l6 cells: their effects on host cell proliferation and cell cycle changes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870968/
https://www.ncbi.nlm.nih.gov/pubmed/27180572
http://dx.doi.org/10.3347/kjp.2016.54.2.147
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