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Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser

We report on a miniature fiber-optic multiphoton microscopy (MPM) system based on a frequency-doubled femtosecond Er-doped fiber laser. The femtosecond pulses from the laser source are delivered to the miniature fiber-optic probe at 1.58 µm wavelength, where a standard single mode fiber is used for...

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Detalles Bibliográficos
Autores principales: Huang, Lin, Mills, Arthur K., Zhao, Yuan, Jones, David J., Tang, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871093/
https://www.ncbi.nlm.nih.gov/pubmed/27231633
http://dx.doi.org/10.1364/BOE.7.001948
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author Huang, Lin
Mills, Arthur K.
Zhao, Yuan
Jones, David J.
Tang, Shuo
author_facet Huang, Lin
Mills, Arthur K.
Zhao, Yuan
Jones, David J.
Tang, Shuo
author_sort Huang, Lin
collection PubMed
description We report on a miniature fiber-optic multiphoton microscopy (MPM) system based on a frequency-doubled femtosecond Er-doped fiber laser. The femtosecond pulses from the laser source are delivered to the miniature fiber-optic probe at 1.58 µm wavelength, where a standard single mode fiber is used for delivery without the need of free-space dispersion compensation components. The beam is frequency-doubled inside the probe by a periodically poled MgO:LiNbO(3) crystal. Frequency-doubled pulses at 786 nm with a maximum power of 80 mW and a pulsewidth of 150 fs are obtained and applied to excite intrinsic signals from tissues. A MEMS scanner, a miniature objective, and a multimode collection fiber are further used to make the probe compact. The miniature fiber-optic MPM system is highly portable and robust. Ex vivo multiphoton imaging of mammalian skins demonstrates the capability of the system in imaging biological tissues. The results show that the miniature fiber-optic MPM system using frequency-doubled femtosecond fiber laser can potentially bring the MPM imaging for clinical applications.
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spelling pubmed-48710932016-05-26 Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser Huang, Lin Mills, Arthur K. Zhao, Yuan Jones, David J. Tang, Shuo Biomed Opt Express Article We report on a miniature fiber-optic multiphoton microscopy (MPM) system based on a frequency-doubled femtosecond Er-doped fiber laser. The femtosecond pulses from the laser source are delivered to the miniature fiber-optic probe at 1.58 µm wavelength, where a standard single mode fiber is used for delivery without the need of free-space dispersion compensation components. The beam is frequency-doubled inside the probe by a periodically poled MgO:LiNbO(3) crystal. Frequency-doubled pulses at 786 nm with a maximum power of 80 mW and a pulsewidth of 150 fs are obtained and applied to excite intrinsic signals from tissues. A MEMS scanner, a miniature objective, and a multimode collection fiber are further used to make the probe compact. The miniature fiber-optic MPM system is highly portable and robust. Ex vivo multiphoton imaging of mammalian skins demonstrates the capability of the system in imaging biological tissues. The results show that the miniature fiber-optic MPM system using frequency-doubled femtosecond fiber laser can potentially bring the MPM imaging for clinical applications. Optical Society of America 2016-04-19 /pmc/articles/PMC4871093/ /pubmed/27231633 http://dx.doi.org/10.1364/BOE.7.001948 Text en © 2016 Optical Society of America
spellingShingle Article
Huang, Lin
Mills, Arthur K.
Zhao, Yuan
Jones, David J.
Tang, Shuo
Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser
title Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser
title_full Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser
title_fullStr Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser
title_full_unstemmed Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser
title_short Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser
title_sort miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond er-doped fiber laser
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871093/
https://www.ncbi.nlm.nih.gov/pubmed/27231633
http://dx.doi.org/10.1364/BOE.7.001948
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