E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells

Plasmacytoid dendritic cells (pDCs) play important roles in initiating and regulating immune responses. pDC infiltration has been documented in multiple pathological lesions including infections, tumors, and autoimmune diseases, and the severity of pDC infiltration correlates with disease progressio...

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Autores principales: Ma, Jianping, Yu, Haisheng, Yin, Xiangyun, Cheng, Menglan, Shi, Quanxing, Yin, Zhao, Nie, Xiaohua, Shouli, Wang, Zhang, Liguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871903/
https://www.ncbi.nlm.nih.gov/pubmed/27340692
http://dx.doi.org/10.1007/s41048-016-0023-6
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author Ma, Jianping
Yu, Haisheng
Yin, Xiangyun
Cheng, Menglan
Shi, Quanxing
Yin, Zhao
Nie, Xiaohua
Shouli, Wang
Zhang, Liguo
author_facet Ma, Jianping
Yu, Haisheng
Yin, Xiangyun
Cheng, Menglan
Shi, Quanxing
Yin, Zhao
Nie, Xiaohua
Shouli, Wang
Zhang, Liguo
author_sort Ma, Jianping
collection PubMed
description Plasmacytoid dendritic cells (pDCs) play important roles in initiating and regulating immune responses. pDC infiltration has been documented in multiple pathological lesions including infections, tumors, and autoimmune diseases, and the severity of pDC infiltration correlates with disease progression. However, a specific antibody for identifying pDCs by immunohistochemical staining on paraffin-embedded tissue sections is still lacking. Here, we developed a novel antibody targeted E2-2, a transcription factor preferentially expressed in pDCs. The antibody stains the nuclei of pDCs specifically in immunohistochemical analysis of various tissues from both human and rhesus monkey. This novel antibody will serve as a beneficial tool for pDC-related basic research and clinical investigation.
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spelling pubmed-48719032016-06-21 E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells Ma, Jianping Yu, Haisheng Yin, Xiangyun Cheng, Menglan Shi, Quanxing Yin, Zhao Nie, Xiaohua Shouli, Wang Zhang, Liguo Biophys Rep Methods Plasmacytoid dendritic cells (pDCs) play important roles in initiating and regulating immune responses. pDC infiltration has been documented in multiple pathological lesions including infections, tumors, and autoimmune diseases, and the severity of pDC infiltration correlates with disease progression. However, a specific antibody for identifying pDCs by immunohistochemical staining on paraffin-embedded tissue sections is still lacking. Here, we developed a novel antibody targeted E2-2, a transcription factor preferentially expressed in pDCs. The antibody stains the nuclei of pDCs specifically in immunohistochemical analysis of various tissues from both human and rhesus monkey. This novel antibody will serve as a beneficial tool for pDC-related basic research and clinical investigation. Springer Berlin Heidelberg 2016-04-11 2015 /pmc/articles/PMC4871903/ /pubmed/27340692 http://dx.doi.org/10.1007/s41048-016-0023-6 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Methods
Ma, Jianping
Yu, Haisheng
Yin, Xiangyun
Cheng, Menglan
Shi, Quanxing
Yin, Zhao
Nie, Xiaohua
Shouli, Wang
Zhang, Liguo
E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
title E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
title_full E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
title_fullStr E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
title_full_unstemmed E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
title_short E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
title_sort e2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells
topic Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871903/
https://www.ncbi.nlm.nih.gov/pubmed/27340692
http://dx.doi.org/10.1007/s41048-016-0023-6
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