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Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions
Management of phosphorus discharge from human waste is essential for the control of eutrophication in surface waters. Enhanced biological phosphorus removal (EBPR) is a sustainable, efficient way of removing phosphorus from waste water without employing chemical precipitation, but is assumed unachie...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872125/ https://www.ncbi.nlm.nih.gov/pubmed/27193869 http://dx.doi.org/10.1038/srep25719 |
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author | Law, Yingyu Kirkegaard, Rasmus Hansen Cokro, Angel Anisa Liu, Xianghui Arumugam, Krithika Xie, Chao Stokholm-Bjerregaard, Mikkel Drautz-Moses, Daniela I. Nielsen, Per Halkjær Wuertz, Stefan Williams, Rohan B. H. |
author_facet | Law, Yingyu Kirkegaard, Rasmus Hansen Cokro, Angel Anisa Liu, Xianghui Arumugam, Krithika Xie, Chao Stokholm-Bjerregaard, Mikkel Drautz-Moses, Daniela I. Nielsen, Per Halkjær Wuertz, Stefan Williams, Rohan B. H. |
author_sort | Law, Yingyu |
collection | PubMed |
description | Management of phosphorus discharge from human waste is essential for the control of eutrophication in surface waters. Enhanced biological phosphorus removal (EBPR) is a sustainable, efficient way of removing phosphorus from waste water without employing chemical precipitation, but is assumed unachievable in tropical temperatures due to conditions that favour glycogen accumulating organisms (GAOs) over polyphosphate accumulating organisms (PAOs). Here, we show these assumptions are unfounded by studying comparative community dynamics in a full-scale plant following systematic perturbation of operational conditions, which modified community abundance, function and physicochemical state. A statistically significant increase in the relative abundance of the PAO Accumulibacter was associated with improved EBPR activity. GAO relative abundance also increased, challenging the assumption of competition. An Accumulibacter bin-genome was identified from a whole community metagenomic survey, and comparative analysis against extant Accumulibacter genomes suggests a close relationship to Type II. Analysis of the associated metatranscriptome data revealed that genes encoding proteins involved in the tricarboxylic acid cycle and glycolysis pathways were highly expressed, consistent with metabolic modelling results. Our findings show that tropical EBPR is indeed possible, highlight the translational potential of studying competition dynamics in full-scale waste water communities and carry implications for plant design in tropical regions. |
format | Online Article Text |
id | pubmed-4872125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48721252016-06-01 Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions Law, Yingyu Kirkegaard, Rasmus Hansen Cokro, Angel Anisa Liu, Xianghui Arumugam, Krithika Xie, Chao Stokholm-Bjerregaard, Mikkel Drautz-Moses, Daniela I. Nielsen, Per Halkjær Wuertz, Stefan Williams, Rohan B. H. Sci Rep Article Management of phosphorus discharge from human waste is essential for the control of eutrophication in surface waters. Enhanced biological phosphorus removal (EBPR) is a sustainable, efficient way of removing phosphorus from waste water without employing chemical precipitation, but is assumed unachievable in tropical temperatures due to conditions that favour glycogen accumulating organisms (GAOs) over polyphosphate accumulating organisms (PAOs). Here, we show these assumptions are unfounded by studying comparative community dynamics in a full-scale plant following systematic perturbation of operational conditions, which modified community abundance, function and physicochemical state. A statistically significant increase in the relative abundance of the PAO Accumulibacter was associated with improved EBPR activity. GAO relative abundance also increased, challenging the assumption of competition. An Accumulibacter bin-genome was identified from a whole community metagenomic survey, and comparative analysis against extant Accumulibacter genomes suggests a close relationship to Type II. Analysis of the associated metatranscriptome data revealed that genes encoding proteins involved in the tricarboxylic acid cycle and glycolysis pathways were highly expressed, consistent with metabolic modelling results. Our findings show that tropical EBPR is indeed possible, highlight the translational potential of studying competition dynamics in full-scale waste water communities and carry implications for plant design in tropical regions. Nature Publishing Group 2016-05-19 /pmc/articles/PMC4872125/ /pubmed/27193869 http://dx.doi.org/10.1038/srep25719 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Law, Yingyu Kirkegaard, Rasmus Hansen Cokro, Angel Anisa Liu, Xianghui Arumugam, Krithika Xie, Chao Stokholm-Bjerregaard, Mikkel Drautz-Moses, Daniela I. Nielsen, Per Halkjær Wuertz, Stefan Williams, Rohan B. H. Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
title | Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
title_full | Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
title_fullStr | Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
title_full_unstemmed | Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
title_short | Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
title_sort | integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872125/ https://www.ncbi.nlm.nih.gov/pubmed/27193869 http://dx.doi.org/10.1038/srep25719 |
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