Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells

The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human...

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Detalles Bibliográficos
Autores principales: Le Tonquèze, Olivier, Gschloessl, Bernhard, Legagneux, Vincent, Paillard, Luc, Audic, Yann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872370/
https://www.ncbi.nlm.nih.gov/pubmed/27222809
http://dx.doi.org/10.1016/j.gdata.2016.04.009
Descripción
Sumario:The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human HeLa cells. The CELF1 binding sites deduced from the sequence data allow characterizing specific features of CELF1-RNA association. We present therefore the first map of CELF1 binding sites in human cells.

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