Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells

The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human...

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Autores principales: Le Tonquèze, Olivier, Gschloessl, Bernhard, Legagneux, Vincent, Paillard, Luc, Audic, Yann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872370/
https://www.ncbi.nlm.nih.gov/pubmed/27222809
http://dx.doi.org/10.1016/j.gdata.2016.04.009
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author Le Tonquèze, Olivier
Gschloessl, Bernhard
Legagneux, Vincent
Paillard, Luc
Audic, Yann
author_facet Le Tonquèze, Olivier
Gschloessl, Bernhard
Legagneux, Vincent
Paillard, Luc
Audic, Yann
author_sort Le Tonquèze, Olivier
collection PubMed
description The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human HeLa cells. The CELF1 binding sites deduced from the sequence data allow characterizing specific features of CELF1-RNA association. We present therefore the first map of CELF1 binding sites in human cells.
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spelling pubmed-48723702016-05-24 Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells Le Tonquèze, Olivier Gschloessl, Bernhard Legagneux, Vincent Paillard, Luc Audic, Yann Genom Data Regular Article The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human HeLa cells. The CELF1 binding sites deduced from the sequence data allow characterizing specific features of CELF1-RNA association. We present therefore the first map of CELF1 binding sites in human cells. Elsevier 2016-04-19 /pmc/articles/PMC4872370/ /pubmed/27222809 http://dx.doi.org/10.1016/j.gdata.2016.04.009 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Article
Le Tonquèze, Olivier
Gschloessl, Bernhard
Legagneux, Vincent
Paillard, Luc
Audic, Yann
Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells
title Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells
title_full Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells
title_fullStr Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells
title_full_unstemmed Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells
title_short Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells
title_sort identification of celf1 rna targets by clip-seq in human hela cells
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4872370/
https://www.ncbi.nlm.nih.gov/pubmed/27222809
http://dx.doi.org/10.1016/j.gdata.2016.04.009
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