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Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola
The white pine blister rust (WPBR) fungus Cronartium ribicola (J.C. Fisch.) is an exotic invasive forest pathogen causing severe stem canker disease of native white pine trees (subgenus Strobus) in North America. The present study reports discovery of five novel mitoviruses in C. ribicola by deep RN...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873031/ https://www.ncbi.nlm.nih.gov/pubmed/27196406 http://dx.doi.org/10.1371/journal.pone.0154267 |
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author | Liu, Jun-Jun Chan, Danelle Xiang, Yu Williams, Holly Li, Xiao-Rui Sniezko, Richard A. Sturrock, Rona N. |
author_facet | Liu, Jun-Jun Chan, Danelle Xiang, Yu Williams, Holly Li, Xiao-Rui Sniezko, Richard A. Sturrock, Rona N. |
author_sort | Liu, Jun-Jun |
collection | PubMed |
description | The white pine blister rust (WPBR) fungus Cronartium ribicola (J.C. Fisch.) is an exotic invasive forest pathogen causing severe stem canker disease of native white pine trees (subgenus Strobus) in North America. The present study reports discovery of five novel mitoviruses in C. ribicola by deep RNA sequencing. The complete genome of each mitovirus was determined by rapid amplification of cDNA ends (RACE) and reverse transcriptase-polymerase chain reaction (RT-PCR). A single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) was detected in each of the viral genomes using mitochondrial genetic codes. Phylogenetic analysis indicated that the C. ribicola mitoviruses (CrMV1 to CrMV5) are new putative species of the genus Mitovirus. qRT-PCR and RNA-Seq analyses revealed that viral RNAs were significantly increased in fungal mycelia in cankered pine stems compared to expression during two different stages of spore development, suggesting that viral genome replication and transcription benefit from active growth of the host fungus. CrMVs were widespread with relatively high levels of minor allele frequency (MAF) in western North America. As the first report of mitoviruses in the Class Pucciniomycetes, this work allows further investigation of the dynamics of a viral community in the WPBR pathosystem, including potential impacts that may affect pathogenicity and virulence of the host fungus. |
format | Online Article Text |
id | pubmed-4873031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48730312016-06-09 Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola Liu, Jun-Jun Chan, Danelle Xiang, Yu Williams, Holly Li, Xiao-Rui Sniezko, Richard A. Sturrock, Rona N. PLoS One Research Article The white pine blister rust (WPBR) fungus Cronartium ribicola (J.C. Fisch.) is an exotic invasive forest pathogen causing severe stem canker disease of native white pine trees (subgenus Strobus) in North America. The present study reports discovery of five novel mitoviruses in C. ribicola by deep RNA sequencing. The complete genome of each mitovirus was determined by rapid amplification of cDNA ends (RACE) and reverse transcriptase-polymerase chain reaction (RT-PCR). A single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) was detected in each of the viral genomes using mitochondrial genetic codes. Phylogenetic analysis indicated that the C. ribicola mitoviruses (CrMV1 to CrMV5) are new putative species of the genus Mitovirus. qRT-PCR and RNA-Seq analyses revealed that viral RNAs were significantly increased in fungal mycelia in cankered pine stems compared to expression during two different stages of spore development, suggesting that viral genome replication and transcription benefit from active growth of the host fungus. CrMVs were widespread with relatively high levels of minor allele frequency (MAF) in western North America. As the first report of mitoviruses in the Class Pucciniomycetes, this work allows further investigation of the dynamics of a viral community in the WPBR pathosystem, including potential impacts that may affect pathogenicity and virulence of the host fungus. Public Library of Science 2016-05-19 /pmc/articles/PMC4873031/ /pubmed/27196406 http://dx.doi.org/10.1371/journal.pone.0154267 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Liu, Jun-Jun Chan, Danelle Xiang, Yu Williams, Holly Li, Xiao-Rui Sniezko, Richard A. Sturrock, Rona N. Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola |
title | Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola |
title_full | Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola |
title_fullStr | Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola |
title_full_unstemmed | Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola |
title_short | Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola |
title_sort | characterization of five novel mitoviruses in the white pine blister rust fungus cronartium ribicola |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873031/ https://www.ncbi.nlm.nih.gov/pubmed/27196406 http://dx.doi.org/10.1371/journal.pone.0154267 |
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