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Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice

Members of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore proper...

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Autores principales: Wu, Xudong, Indzhykulian, Artur A., Niksch, Paul D., Webber, Roxanna M., Garcia-Gonzalez, Miguel, Watnick, Terry, Zhou, Jing, Vollrath, Melissa A., Corey, David P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873267/
https://www.ncbi.nlm.nih.gov/pubmed/27196058
http://dx.doi.org/10.1371/journal.pone.0155577
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author Wu, Xudong
Indzhykulian, Artur A.
Niksch, Paul D.
Webber, Roxanna M.
Garcia-Gonzalez, Miguel
Watnick, Terry
Zhou, Jing
Vollrath, Melissa A.
Corey, David P.
author_facet Wu, Xudong
Indzhykulian, Artur A.
Niksch, Paul D.
Webber, Roxanna M.
Garcia-Gonzalez, Miguel
Watnick, Terry
Zhou, Jing
Vollrath, Melissa A.
Corey, David P.
author_sort Wu, Xudong
collection PubMed
description Members of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore properties not similar to the hair-cell channel. Such studies have excluded Trpv4, Trpa1, Trpml3, Trpm1, Trpm3, Trpc1, Trpc3, Trpc5, and Trpc6. However, others remain reasonable candidates. We used data from an RNA-seq analysis of gene expression in hair cells as well as data on TRP channel conductance to narrow the candidate group. We then characterized mice lacking functional Trpm2, Pkd2, Pkd2l1, Pkd2l2 and Pkd1l3, using scanning electron microscopy, auditory brainstem response, permeant dye accumulation, and single-cell electrophysiology. In all of these TRP-deficient mice, and in double and triple knockouts, mechanotransduction persisted. Together with published studies, these results argue against the participation of any of the 33 mouse TRP channels in hair cell transduction.
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spelling pubmed-48732672016-06-09 Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice Wu, Xudong Indzhykulian, Artur A. Niksch, Paul D. Webber, Roxanna M. Garcia-Gonzalez, Miguel Watnick, Terry Zhou, Jing Vollrath, Melissa A. Corey, David P. PLoS One Research Article Members of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore properties not similar to the hair-cell channel. Such studies have excluded Trpv4, Trpa1, Trpml3, Trpm1, Trpm3, Trpc1, Trpc3, Trpc5, and Trpc6. However, others remain reasonable candidates. We used data from an RNA-seq analysis of gene expression in hair cells as well as data on TRP channel conductance to narrow the candidate group. We then characterized mice lacking functional Trpm2, Pkd2, Pkd2l1, Pkd2l2 and Pkd1l3, using scanning electron microscopy, auditory brainstem response, permeant dye accumulation, and single-cell electrophysiology. In all of these TRP-deficient mice, and in double and triple knockouts, mechanotransduction persisted. Together with published studies, these results argue against the participation of any of the 33 mouse TRP channels in hair cell transduction. Public Library of Science 2016-05-19 /pmc/articles/PMC4873267/ /pubmed/27196058 http://dx.doi.org/10.1371/journal.pone.0155577 Text en © 2016 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wu, Xudong
Indzhykulian, Artur A.
Niksch, Paul D.
Webber, Roxanna M.
Garcia-Gonzalez, Miguel
Watnick, Terry
Zhou, Jing
Vollrath, Melissa A.
Corey, David P.
Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice
title Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice
title_full Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice
title_fullStr Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice
title_full_unstemmed Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice
title_short Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice
title_sort hair-cell mechanotransduction persists in trp channel knockout mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873267/
https://www.ncbi.nlm.nih.gov/pubmed/27196058
http://dx.doi.org/10.1371/journal.pone.0155577
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