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Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)

PREMISE OF THE STUDY: Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species. METHODS AND RESULTS: Using next-...

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Autores principales: Sugai, Kyoko, Watanabe, Kenta, Kato, Hidetoshi, Sugawara, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873269/
https://www.ncbi.nlm.nih.gov/pubmed/27213122
http://dx.doi.org/10.3732/apps.1500133
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author Sugai, Kyoko
Watanabe, Kenta
Kato, Hidetoshi
Sugawara, Takashi
author_facet Sugai, Kyoko
Watanabe, Kenta
Kato, Hidetoshi
Sugawara, Takashi
author_sort Sugai, Kyoko
collection PubMed
description PREMISE OF THE STUDY: Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species. METHODS AND RESULTS: Using next-generation sequencing, we developed 26 SSR markers for P. homalosperma with perfect motifs from di- to pentanucleotide repeats. Of these, the Chichijima and Hahajima island populations of P. homalosperma had mean allele numbers of 6.50 and 6.81, respectively. The mean expected heterozygosities were 0.578 and 0.606, respectively. In addition, 10 and eight of these markers were successfully amplified for P. boninensis and P. serpens, respectively, occurring in the same or adjacent areas. CONCLUSIONS: The SSR markers developed in this study will be useful for future studies concerning the population genetics of P. homalosperma and will facilitate the development of a conservation strategy.
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spelling pubmed-48732692016-05-20 Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) Sugai, Kyoko Watanabe, Kenta Kato, Hidetoshi Sugawara, Takashi Appl Plant Sci Primer Note PREMISE OF THE STUDY: Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species. METHODS AND RESULTS: Using next-generation sequencing, we developed 26 SSR markers for P. homalosperma with perfect motifs from di- to pentanucleotide repeats. Of these, the Chichijima and Hahajima island populations of P. homalosperma had mean allele numbers of 6.50 and 6.81, respectively. The mean expected heterozygosities were 0.578 and 0.606, respectively. In addition, 10 and eight of these markers were successfully amplified for P. boninensis and P. serpens, respectively, occurring in the same or adjacent areas. CONCLUSIONS: The SSR markers developed in this study will be useful for future studies concerning the population genetics of P. homalosperma and will facilitate the development of a conservation strategy. Botanical Society of America 2016-05-06 /pmc/articles/PMC4873269/ /pubmed/27213122 http://dx.doi.org/10.3732/apps.1500133 Text en © 2016 Sugai et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Sugai, Kyoko
Watanabe, Kenta
Kato, Hidetoshi
Sugawara, Takashi
Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
title Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
title_full Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
title_fullStr Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
title_full_unstemmed Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
title_short Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
title_sort development of ssr markers for psychotria homalosperma (rubiaceae) and cross-amplification in four other species(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873269/
https://www.ncbi.nlm.nih.gov/pubmed/27213122
http://dx.doi.org/10.3732/apps.1500133
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