Cargando…
Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1)
PREMISE OF THE STUDY: Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species. METHODS AND RESULTS: Using next-...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873269/ https://www.ncbi.nlm.nih.gov/pubmed/27213122 http://dx.doi.org/10.3732/apps.1500133 |
_version_ | 1782432875854954496 |
---|---|
author | Sugai, Kyoko Watanabe, Kenta Kato, Hidetoshi Sugawara, Takashi |
author_facet | Sugai, Kyoko Watanabe, Kenta Kato, Hidetoshi Sugawara, Takashi |
author_sort | Sugai, Kyoko |
collection | PubMed |
description | PREMISE OF THE STUDY: Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species. METHODS AND RESULTS: Using next-generation sequencing, we developed 26 SSR markers for P. homalosperma with perfect motifs from di- to pentanucleotide repeats. Of these, the Chichijima and Hahajima island populations of P. homalosperma had mean allele numbers of 6.50 and 6.81, respectively. The mean expected heterozygosities were 0.578 and 0.606, respectively. In addition, 10 and eight of these markers were successfully amplified for P. boninensis and P. serpens, respectively, occurring in the same or adjacent areas. CONCLUSIONS: The SSR markers developed in this study will be useful for future studies concerning the population genetics of P. homalosperma and will facilitate the development of a conservation strategy. |
format | Online Article Text |
id | pubmed-4873269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-48732692016-05-20 Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) Sugai, Kyoko Watanabe, Kenta Kato, Hidetoshi Sugawara, Takashi Appl Plant Sci Primer Note PREMISE OF THE STUDY: Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species. METHODS AND RESULTS: Using next-generation sequencing, we developed 26 SSR markers for P. homalosperma with perfect motifs from di- to pentanucleotide repeats. Of these, the Chichijima and Hahajima island populations of P. homalosperma had mean allele numbers of 6.50 and 6.81, respectively. The mean expected heterozygosities were 0.578 and 0.606, respectively. In addition, 10 and eight of these markers were successfully amplified for P. boninensis and P. serpens, respectively, occurring in the same or adjacent areas. CONCLUSIONS: The SSR markers developed in this study will be useful for future studies concerning the population genetics of P. homalosperma and will facilitate the development of a conservation strategy. Botanical Society of America 2016-05-06 /pmc/articles/PMC4873269/ /pubmed/27213122 http://dx.doi.org/10.3732/apps.1500133 Text en © 2016 Sugai et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Sugai, Kyoko Watanabe, Kenta Kato, Hidetoshi Sugawara, Takashi Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) |
title | Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) |
title_full | Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) |
title_fullStr | Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) |
title_full_unstemmed | Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) |
title_short | Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species(1) |
title_sort | development of ssr markers for psychotria homalosperma (rubiaceae) and cross-amplification in four other species(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873269/ https://www.ncbi.nlm.nih.gov/pubmed/27213122 http://dx.doi.org/10.3732/apps.1500133 |
work_keys_str_mv | AT sugaikyoko developmentofssrmarkersforpsychotriahomalospermarubiaceaeandcrossamplificationinfourotherspecies1 AT watanabekenta developmentofssrmarkersforpsychotriahomalospermarubiaceaeandcrossamplificationinfourotherspecies1 AT katohidetoshi developmentofssrmarkersforpsychotriahomalospermarubiaceaeandcrossamplificationinfourotherspecies1 AT sugawaratakashi developmentofssrmarkersforpsychotriahomalospermarubiaceaeandcrossamplificationinfourotherspecies1 |