Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)

PREMISE OF THE STUDY: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. ME...

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Detalles Bibliográficos
Autores principales: Morillo, Eduardo, Buitron, Johanna, Limongi, Ricardo, Vignes, Helene, Argout, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873270/
https://www.ncbi.nlm.nih.gov/pubmed/27213123
http://dx.doi.org/10.3732/apps.1500135
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author Morillo, Eduardo
Buitron, Johanna
Limongi, Ricardo
Vignes, Helene
Argout, Xavier
author_facet Morillo, Eduardo
Buitron, Johanna
Limongi, Ricardo
Vignes, Helene
Argout, Xavier
author_sort Morillo, Eduardo
collection PubMed
description PREMISE OF THE STUDY: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. METHODS AND RESULTS: Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii. CONCLUSIONS: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.
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spelling pubmed-48732702016-05-20 Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1) Morillo, Eduardo Buitron, Johanna Limongi, Ricardo Vignes, Helene Argout, Xavier Appl Plant Sci Primer Note PREMISE OF THE STUDY: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. METHODS AND RESULTS: Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii. CONCLUSIONS: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species. Botanical Society of America 2016-05-09 /pmc/articles/PMC4873270/ /pubmed/27213123 http://dx.doi.org/10.3732/apps.1500135 Text en © 2016 Morillo et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Morillo, Eduardo
Buitron, Johanna
Limongi, Ricardo
Vignes, Helene
Argout, Xavier
Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)
title Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)
title_full Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)
title_fullStr Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)
title_full_unstemmed Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)
title_short Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)(1)
title_sort characterization of microsatellites identified by next-generation sequencing in the neotropical tree handroanthus billbergii (bignoniaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873270/
https://www.ncbi.nlm.nih.gov/pubmed/27213123
http://dx.doi.org/10.3732/apps.1500135
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