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Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation

Phasins are important proteins controlling poly-3-hydroxybutyrate (PHB) granules formation, their number into the cell and stability. The genome sequencing of the endophytic and diazotrophic bacterium Herbaspirillum seropedicae SmR1 revealed two homologous phasin genes. To verify the role of the pha...

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Autores principales: Alves, Luis P. S., Teixeira, Cícero S., Tirapelle, Evandro F., Donatti, Lucélia, Tadra-Sfeir, Michelle Z., Steffens, Maria B. R., de Souza, Emanuel M., de Oliveira Pedrosa, Fabio, Chubatsu, Leda S., Müller-Santos, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873508/
https://www.ncbi.nlm.nih.gov/pubmed/27242754
http://dx.doi.org/10.3389/fmicb.2016.00739
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author Alves, Luis P. S.
Teixeira, Cícero S.
Tirapelle, Evandro F.
Donatti, Lucélia
Tadra-Sfeir, Michelle Z.
Steffens, Maria B. R.
de Souza, Emanuel M.
de Oliveira Pedrosa, Fabio
Chubatsu, Leda S.
Müller-Santos, Marcelo
author_facet Alves, Luis P. S.
Teixeira, Cícero S.
Tirapelle, Evandro F.
Donatti, Lucélia
Tadra-Sfeir, Michelle Z.
Steffens, Maria B. R.
de Souza, Emanuel M.
de Oliveira Pedrosa, Fabio
Chubatsu, Leda S.
Müller-Santos, Marcelo
author_sort Alves, Luis P. S.
collection PubMed
description Phasins are important proteins controlling poly-3-hydroxybutyrate (PHB) granules formation, their number into the cell and stability. The genome sequencing of the endophytic and diazotrophic bacterium Herbaspirillum seropedicae SmR1 revealed two homologous phasin genes. To verify the role of the phasins on PHB accumulation in the parental strain H. seropedicae SmR1, isogenic strains defective in the expression of phaP1, phaP2 or both genes were obtained by gene deletion and characterized in this work. Despite of the high sequence similarity between PhaP1 and PhaP2, PhaP1 is the major phasin in H. seropedicae, since its deletion reduced PHB accumulation by ≈50% in comparison to the parental and ΔphaP2. Upon deletion of phaP1, the expression of phaP2 was sixfold enhanced in the ΔphaP1 strain. The responsive backup expression of phaP2 partially rescued the ΔphaP1 mutant, maintaining about 50% of the parental PHB level. The double mutant ΔphaP1.2 did not accumulate PHB in any growth stage and showed a severe reduction of growth when glucose was the carbon source, a clear demonstration of negative impact in the fitness. The co-occurrence of phaP1 and phaP2 homologous in bacteria relatives of H. seropedicae, including other endophytes, indicates that the mechanism of phasin compensation by phaP2 expression may be operating in other organisms, showing that PHB metabolism is a key factor to adaptation and efficiency of endophytic bacteria.
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spelling pubmed-48735082016-05-30 Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation Alves, Luis P. S. Teixeira, Cícero S. Tirapelle, Evandro F. Donatti, Lucélia Tadra-Sfeir, Michelle Z. Steffens, Maria B. R. de Souza, Emanuel M. de Oliveira Pedrosa, Fabio Chubatsu, Leda S. Müller-Santos, Marcelo Front Microbiol Microbiology Phasins are important proteins controlling poly-3-hydroxybutyrate (PHB) granules formation, their number into the cell and stability. The genome sequencing of the endophytic and diazotrophic bacterium Herbaspirillum seropedicae SmR1 revealed two homologous phasin genes. To verify the role of the phasins on PHB accumulation in the parental strain H. seropedicae SmR1, isogenic strains defective in the expression of phaP1, phaP2 or both genes were obtained by gene deletion and characterized in this work. Despite of the high sequence similarity between PhaP1 and PhaP2, PhaP1 is the major phasin in H. seropedicae, since its deletion reduced PHB accumulation by ≈50% in comparison to the parental and ΔphaP2. Upon deletion of phaP1, the expression of phaP2 was sixfold enhanced in the ΔphaP1 strain. The responsive backup expression of phaP2 partially rescued the ΔphaP1 mutant, maintaining about 50% of the parental PHB level. The double mutant ΔphaP1.2 did not accumulate PHB in any growth stage and showed a severe reduction of growth when glucose was the carbon source, a clear demonstration of negative impact in the fitness. The co-occurrence of phaP1 and phaP2 homologous in bacteria relatives of H. seropedicae, including other endophytes, indicates that the mechanism of phasin compensation by phaP2 expression may be operating in other organisms, showing that PHB metabolism is a key factor to adaptation and efficiency of endophytic bacteria. Frontiers Media S.A. 2016-05-20 /pmc/articles/PMC4873508/ /pubmed/27242754 http://dx.doi.org/10.3389/fmicb.2016.00739 Text en Copyright © 2016 Alves, Teixeira, Tirapelle, Donatti, Tadra-Sfeir, Steffens, de Souza, de Oliveira Pedrosa, Chubatsu and Müller-Santos. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Alves, Luis P. S.
Teixeira, Cícero S.
Tirapelle, Evandro F.
Donatti, Lucélia
Tadra-Sfeir, Michelle Z.
Steffens, Maria B. R.
de Souza, Emanuel M.
de Oliveira Pedrosa, Fabio
Chubatsu, Leda S.
Müller-Santos, Marcelo
Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation
title Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation
title_full Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation
title_fullStr Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation
title_full_unstemmed Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation
title_short Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation
title_sort backup expression of the phap2 phasin compensates for phap1 deletion in herbaspirillum seropedicae, maintaining fitness and phb accumulation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873508/
https://www.ncbi.nlm.nih.gov/pubmed/27242754
http://dx.doi.org/10.3389/fmicb.2016.00739
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