EC-QCL mid-IR transmission spectroscopy for monitoring dynamic changes of protein secondary structure in aqueous solution on the example of β-aggregation in alcohol-denaturated α-chymotrypsin

In this work, a novel EC-QCL-based setup for mid-IR transmission measurements in the amide I region is introduced for monitoring dynamic changes in secondary structure of proteins. For this purpose, α-chymotrypsin (aCT) acts as a model protein, which gradually forms intermolecular β-sheet aggregates...

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Detalles Bibliográficos
Autores principales: Alcaráz, Mirta R., Schwaighofer, Andreas, Goicoechea, Héctor, Lendl, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873525/
https://www.ncbi.nlm.nih.gov/pubmed/27007739
http://dx.doi.org/10.1007/s00216-016-9464-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873525/
https://www.ncbi.nlm.nih.gov/pubmed/27007739
http://dx.doi.org/10.1007/s00216-016-9464-5

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