Cargando…

Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy

The insulin/insulin-like growth factor signaling pathway is evolutionarily conserved in animals, and is part of nutrient-sensing mechanisms that control growth, metabolism, reproduction, stress responses, and lifespan. In Drosophila, eight insulin-like peptides (DILP1-8) are known, six of which have...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yiting, Liao, Sifang, Veenstra, Jan A., Nässel, Dick R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873736/
https://www.ncbi.nlm.nih.gov/pubmed/27197757
http://dx.doi.org/10.1038/srep26620
_version_ 1782432933217304576
author Liu, Yiting
Liao, Sifang
Veenstra, Jan A.
Nässel, Dick R.
author_facet Liu, Yiting
Liao, Sifang
Veenstra, Jan A.
Nässel, Dick R.
author_sort Liu, Yiting
collection PubMed
description The insulin/insulin-like growth factor signaling pathway is evolutionarily conserved in animals, and is part of nutrient-sensing mechanisms that control growth, metabolism, reproduction, stress responses, and lifespan. In Drosophila, eight insulin-like peptides (DILP1-8) are known, six of which have been investigated in some detail, whereas expression and functions of DILP1 and DILP4 remain enigmatic. Here we demonstrate that dilp1/DILP1 is transiently expressed in brain insulin producing cells (IPCs) from early pupa until a few days of adult life. However, in adult female flies where diapause is triggered by low temperature and short days, within a time window 0–10h post-eclosion, the dilp1/DILP1 expression remains high for at least 9 weeks. The dilp1 mRNA level is increased in dilp2, 3, 5 and dilp6 mutant flies, indicating feedback regulation. Furthermore, the DILP1 expression in IPCs is regulated by short neuropeptide F, juvenile hormone and presence of larval adipocytes. Male dilp1 mutant flies display increased lifespan and reduced starvation resistance, whereas in female dilp1 mutants oviposition is reduced. Thus, DILP1 is expressed in non-feeding stages and in diapausing flies, is under feedback regulation and appears to play sex-specific functional roles.
format Online
Article
Text
id pubmed-4873736
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-48737362016-06-02 Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy Liu, Yiting Liao, Sifang Veenstra, Jan A. Nässel, Dick R. Sci Rep Article The insulin/insulin-like growth factor signaling pathway is evolutionarily conserved in animals, and is part of nutrient-sensing mechanisms that control growth, metabolism, reproduction, stress responses, and lifespan. In Drosophila, eight insulin-like peptides (DILP1-8) are known, six of which have been investigated in some detail, whereas expression and functions of DILP1 and DILP4 remain enigmatic. Here we demonstrate that dilp1/DILP1 is transiently expressed in brain insulin producing cells (IPCs) from early pupa until a few days of adult life. However, in adult female flies where diapause is triggered by low temperature and short days, within a time window 0–10h post-eclosion, the dilp1/DILP1 expression remains high for at least 9 weeks. The dilp1 mRNA level is increased in dilp2, 3, 5 and dilp6 mutant flies, indicating feedback regulation. Furthermore, the DILP1 expression in IPCs is regulated by short neuropeptide F, juvenile hormone and presence of larval adipocytes. Male dilp1 mutant flies display increased lifespan and reduced starvation resistance, whereas in female dilp1 mutants oviposition is reduced. Thus, DILP1 is expressed in non-feeding stages and in diapausing flies, is under feedback regulation and appears to play sex-specific functional roles. Nature Publishing Group 2016-05-20 /pmc/articles/PMC4873736/ /pubmed/27197757 http://dx.doi.org/10.1038/srep26620 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Yiting
Liao, Sifang
Veenstra, Jan A.
Nässel, Dick R.
Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
title Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
title_full Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
title_fullStr Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
title_full_unstemmed Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
title_short Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy
title_sort drosophila insulin-like peptide 1 (dilp1) is transiently expressed during non-feeding stages and reproductive dormancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873736/
https://www.ncbi.nlm.nih.gov/pubmed/27197757
http://dx.doi.org/10.1038/srep26620
work_keys_str_mv AT liuyiting drosophilainsulinlikepeptide1dilp1istransientlyexpressedduringnonfeedingstagesandreproductivedormancy
AT liaosifang drosophilainsulinlikepeptide1dilp1istransientlyexpressedduringnonfeedingstagesandreproductivedormancy
AT veenstrajana drosophilainsulinlikepeptide1dilp1istransientlyexpressedduringnonfeedingstagesandreproductivedormancy
AT nasseldickr drosophilainsulinlikepeptide1dilp1istransientlyexpressedduringnonfeedingstagesandreproductivedormancy