Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes

Great efforts have been made to develop fluorescent probes for pyrophosphate (PPi) detection. Nucleus staining with fluorescence microscopy has been also widely investigated. But fluorescent probes for PPi detection with high sensitivity in water medium and nucleus staining with low–cost non–preciou...

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Autores principales: Chao, Duobin, Ni, Shitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873787/
https://www.ncbi.nlm.nih.gov/pubmed/27198968
http://dx.doi.org/10.1038/srep26477
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author Chao, Duobin
Ni, Shitan
author_facet Chao, Duobin
Ni, Shitan
author_sort Chao, Duobin
collection PubMed
description Great efforts have been made to develop fluorescent probes for pyrophosphate (PPi) detection. Nucleus staining with fluorescence microscopy has been also widely investigated. But fluorescent probes for PPi detection with high sensitivity in water medium and nucleus staining with low–cost non–precious metal complexes in living cells are still challenging. Herein, we report simple terpyridine–Zn(II) complexes for selective nanomolar PPi detection over ATP and ADP in water based on aggregation induced emission (AIE) and intramolecular charge transfer (ICT). In addition, these terpyridine–Zn(II) complexes were successfully employed for nucleus staining in living cells. These results demonstrated simply obtained terpyridine–Zn(II) complexes are powerful tool for PPi detection and the development of PPi–related studies.
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spelling pubmed-48737872016-06-02 Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes Chao, Duobin Ni, Shitan Sci Rep Article Great efforts have been made to develop fluorescent probes for pyrophosphate (PPi) detection. Nucleus staining with fluorescence microscopy has been also widely investigated. But fluorescent probes for PPi detection with high sensitivity in water medium and nucleus staining with low–cost non–precious metal complexes in living cells are still challenging. Herein, we report simple terpyridine–Zn(II) complexes for selective nanomolar PPi detection over ATP and ADP in water based on aggregation induced emission (AIE) and intramolecular charge transfer (ICT). In addition, these terpyridine–Zn(II) complexes were successfully employed for nucleus staining in living cells. These results demonstrated simply obtained terpyridine–Zn(II) complexes are powerful tool for PPi detection and the development of PPi–related studies. Nature Publishing Group 2016-05-20 /pmc/articles/PMC4873787/ /pubmed/27198968 http://dx.doi.org/10.1038/srep26477 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chao, Duobin
Ni, Shitan
Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
title Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
title_full Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
title_fullStr Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
title_full_unstemmed Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
title_short Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
title_sort nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–zn(ii) complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873787/
https://www.ncbi.nlm.nih.gov/pubmed/27198968
http://dx.doi.org/10.1038/srep26477
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