Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers

Japanese eel endothelial cells-infecting virus (JEECV) has spread in eel farms and caused serious economic loss. In this study, we examined the prevalence of JEECV infection in 100 wild Japanese eel (Anguilla japonica) elvers caught from Yamaguchi prefecture, Japan, using quantitative PCR and conven...

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Detalles Bibliográficos
Autores principales: OKAZAKI, Sachiko, YASUMOTO, Shinya, KOYAMA, Satoshi, TSUCHIAKA, Shinobu, NAOI, Yuki, OMATSU, Tsutomu, ONO, Shin-ichi, MIZUTANI, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Wildlife Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873866/
https://www.ncbi.nlm.nih.gov/pubmed/26672624
http://dx.doi.org/10.1292/jvms.15-0515
Descripción
Sumario:Japanese eel endothelial cells-infecting virus (JEECV) has spread in eel farms and caused serious economic loss. In this study, we examined the prevalence of JEECV infection in 100 wild Japanese eel (Anguilla japonica) elvers caught from Yamaguchi prefecture, Japan, using quantitative PCR and conventional PCR. Total genomic DNA was obtained from the cranial quarter of the body in 70 of 100 eels and from the gill in the remaining. Of 30 gill samples, 20 were analyzed after pooling with other samples, and the remaining 10 were analyzed separately. A single positive result for JEECV was detected following analysis of the 10 separately analyzed samples. This result constitutes the first report of JEECV infection in wild A. japonica elvers.

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