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Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse

Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classic...

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Detalles Bibliográficos
Autores principales: LOONG, Shih Keng, MAHFODZ, Nur Hidayana, WALI, Haryanti Azura Mohamad, TALIB, Siti Aisyah A., NASRAH, Siti Noraisah Ahmad, WONG, Pooi Fong, ABUBAKAR, Sazaly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873868/
https://www.ncbi.nlm.nih.gov/pubmed/26782013
http://dx.doi.org/10.1292/jvms.15-0472
Descripción
Sumario:Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classical Bordetella isolate from the trachea and lung of an ICR mouse. Isolate BH370 was initially identified as B. hinzii by 16S ribosomal DNA and ompA sequencing. Additionally, isolate BH370 also displayed antimicrobial sensitivity profiles similar to B. hinzii. However, analyses of nrdA sequences determined its identity as Bordetella genogroup 16. The isolation of BH370 from a healthy mouse suggests the possibility of it being a commensal. The nrdA gene was demonstrated to possess greater phylogenetic resolution as compared with 16S ribosomal DNA and ompA for the discrimination of non-classical Bordetella species.