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Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse

Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classic...

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Autores principales: LOONG, Shih Keng, MAHFODZ, Nur Hidayana, WALI, Haryanti Azura Mohamad, TALIB, Siti Aisyah A., NASRAH, Siti Noraisah Ahmad, WONG, Pooi Fong, ABUBAKAR, Sazaly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873868/
https://www.ncbi.nlm.nih.gov/pubmed/26782013
http://dx.doi.org/10.1292/jvms.15-0472
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author LOONG, Shih Keng
MAHFODZ, Nur Hidayana
WALI, Haryanti Azura Mohamad
TALIB, Siti Aisyah A.
NASRAH, Siti Noraisah Ahmad
WONG, Pooi Fong
ABUBAKAR, Sazaly
author_facet LOONG, Shih Keng
MAHFODZ, Nur Hidayana
WALI, Haryanti Azura Mohamad
TALIB, Siti Aisyah A.
NASRAH, Siti Noraisah Ahmad
WONG, Pooi Fong
ABUBAKAR, Sazaly
author_sort LOONG, Shih Keng
collection PubMed
description Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classical Bordetella isolate from the trachea and lung of an ICR mouse. Isolate BH370 was initially identified as B. hinzii by 16S ribosomal DNA and ompA sequencing. Additionally, isolate BH370 also displayed antimicrobial sensitivity profiles similar to B. hinzii. However, analyses of nrdA sequences determined its identity as Bordetella genogroup 16. The isolation of BH370 from a healthy mouse suggests the possibility of it being a commensal. The nrdA gene was demonstrated to possess greater phylogenetic resolution as compared with 16S ribosomal DNA and ompA for the discrimination of non-classical Bordetella species.
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spelling pubmed-48738682016-05-25 Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse LOONG, Shih Keng MAHFODZ, Nur Hidayana WALI, Haryanti Azura Mohamad TALIB, Siti Aisyah A. NASRAH, Siti Noraisah Ahmad WONG, Pooi Fong ABUBAKAR, Sazaly J Vet Med Sci Laboratory Animal Science Accurate identification and separation of non-classical Bordetella species is very difficult. These species have been implicated in animal infections. B. hinzii, a non-classical Bordetella, has been isolated from mice in experimental facilities recently. We isolated and characterized one non-classical Bordetella isolate from the trachea and lung of an ICR mouse. Isolate BH370 was initially identified as B. hinzii by 16S ribosomal DNA and ompA sequencing. Additionally, isolate BH370 also displayed antimicrobial sensitivity profiles similar to B. hinzii. However, analyses of nrdA sequences determined its identity as Bordetella genogroup 16. The isolation of BH370 from a healthy mouse suggests the possibility of it being a commensal. The nrdA gene was demonstrated to possess greater phylogenetic resolution as compared with 16S ribosomal DNA and ompA for the discrimination of non-classical Bordetella species. The Japanese Society of Veterinary Science 2016-01-17 2016-04 /pmc/articles/PMC4873868/ /pubmed/26782013 http://dx.doi.org/10.1292/jvms.15-0472 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Laboratory Animal Science
LOONG, Shih Keng
MAHFODZ, Nur Hidayana
WALI, Haryanti Azura Mohamad
TALIB, Siti Aisyah A.
NASRAH, Siti Noraisah Ahmad
WONG, Pooi Fong
ABUBAKAR, Sazaly
Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse
title Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse
title_full Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse
title_fullStr Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse
title_full_unstemmed Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse
title_short Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse
title_sort molecular and antimicrobial analyses of non-classical bordetella isolated from a laboratory mouse
topic Laboratory Animal Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873868/
https://www.ncbi.nlm.nih.gov/pubmed/26782013
http://dx.doi.org/10.1292/jvms.15-0472
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