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Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling

Digital holographic microscopy (DHM) is an emerging imaging technique that permits instantaneous capture of a relatively large sample volume. However, large volumes usually come at the expense of lower spatial resolution, and the technique has rarely been used with prokaryotic cells due to their sma...

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Autores principales: Nadeau, Jay L., Cho, Yong Bin, Kühn, Jonas, Liewer, Kurt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874365/
https://www.ncbi.nlm.nih.gov/pubmed/27242995
http://dx.doi.org/10.3389/fchem.2016.00017
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author Nadeau, Jay L.
Cho, Yong Bin
Kühn, Jonas
Liewer, Kurt
author_facet Nadeau, Jay L.
Cho, Yong Bin
Kühn, Jonas
Liewer, Kurt
author_sort Nadeau, Jay L.
collection PubMed
description Digital holographic microscopy (DHM) is an emerging imaging technique that permits instantaneous capture of a relatively large sample volume. However, large volumes usually come at the expense of lower spatial resolution, and the technique has rarely been used with prokaryotic cells due to their small size and low contrast. In this paper we demonstrate the use of a Mach-Zehnder dual-beam instrument for imaging of labeled and unlabeled bacteria and microalgae. Spatial resolution of 0.3 μm is achieved, providing a sampling of several pixels across a typical prokaryotic cell. Both cellular motility and morphology are readily recorded. The use of dyes provides both amplitude and phase contrast improvement and is of use to identify cells in dense samples.
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spelling pubmed-48743652016-05-30 Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling Nadeau, Jay L. Cho, Yong Bin Kühn, Jonas Liewer, Kurt Front Chem Chemistry Digital holographic microscopy (DHM) is an emerging imaging technique that permits instantaneous capture of a relatively large sample volume. However, large volumes usually come at the expense of lower spatial resolution, and the technique has rarely been used with prokaryotic cells due to their small size and low contrast. In this paper we demonstrate the use of a Mach-Zehnder dual-beam instrument for imaging of labeled and unlabeled bacteria and microalgae. Spatial resolution of 0.3 μm is achieved, providing a sampling of several pixels across a typical prokaryotic cell. Both cellular motility and morphology are readily recorded. The use of dyes provides both amplitude and phase contrast improvement and is of use to identify cells in dense samples. Frontiers Media S.A. 2016-04-19 /pmc/articles/PMC4874365/ /pubmed/27242995 http://dx.doi.org/10.3389/fchem.2016.00017 Text en Copyright © 2016 Nadeau, Cho, Kühn and Liewer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Nadeau, Jay L.
Cho, Yong Bin
Kühn, Jonas
Liewer, Kurt
Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling
title Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling
title_full Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling
title_fullStr Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling
title_full_unstemmed Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling
title_short Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling
title_sort improved tracking and resolution of bacteria in holographic microscopy using dye and fluorescent protein labeling
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874365/
https://www.ncbi.nlm.nih.gov/pubmed/27242995
http://dx.doi.org/10.3389/fchem.2016.00017
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