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Common integration sites of published datasets identified using a graph-based framework

With next-generation sequencing, the genomic data available for the characterization of integration sites (IS) has dramatically increased. At present, in a single experiment, several thousand viral integration genome targets can be investigated to define genomic hot spots. In a previous article, we...

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Autores principales: Vasciaveo, Alessandro, Velevska, Ivana, Politano, Gianfranco, Savino, Alessandro, Schmidt, Manfred, Fronza, Raffaele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874583/
https://www.ncbi.nlm.nih.gov/pubmed/27257471
http://dx.doi.org/10.1016/j.csbj.2015.11.004
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author Vasciaveo, Alessandro
Velevska, Ivana
Politano, Gianfranco
Savino, Alessandro
Schmidt, Manfred
Fronza, Raffaele
author_facet Vasciaveo, Alessandro
Velevska, Ivana
Politano, Gianfranco
Savino, Alessandro
Schmidt, Manfred
Fronza, Raffaele
author_sort Vasciaveo, Alessandro
collection PubMed
description With next-generation sequencing, the genomic data available for the characterization of integration sites (IS) has dramatically increased. At present, in a single experiment, several thousand viral integration genome targets can be investigated to define genomic hot spots. In a previous article, we renovated a formal CIS analysis based on a rigid fixed window demarcation into a more stretchy definition grounded on graphs. Here, we present a selection of supporting data related to the graph-based framework (GBF) from our previous article, in which a collection of common integration sites (CIS) was identified on six published datasets. In this work, we will focus on two datasets, IS(RTCGD) and IS(HIV), which have been previously discussed. Moreover, we show in more detail the workflow design that originates the datasets.
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spelling pubmed-48745832016-06-02 Common integration sites of published datasets identified using a graph-based framework Vasciaveo, Alessandro Velevska, Ivana Politano, Gianfranco Savino, Alessandro Schmidt, Manfred Fronza, Raffaele Comput Struct Biotechnol J Short Communication With next-generation sequencing, the genomic data available for the characterization of integration sites (IS) has dramatically increased. At present, in a single experiment, several thousand viral integration genome targets can be investigated to define genomic hot spots. In a previous article, we renovated a formal CIS analysis based on a rigid fixed window demarcation into a more stretchy definition grounded on graphs. Here, we present a selection of supporting data related to the graph-based framework (GBF) from our previous article, in which a collection of common integration sites (CIS) was identified on six published datasets. In this work, we will focus on two datasets, IS(RTCGD) and IS(HIV), which have been previously discussed. Moreover, we show in more detail the workflow design that originates the datasets. Research Network of Computational and Structural Biotechnology 2015-11-29 /pmc/articles/PMC4874583/ /pubmed/27257471 http://dx.doi.org/10.1016/j.csbj.2015.11.004 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Communication
Vasciaveo, Alessandro
Velevska, Ivana
Politano, Gianfranco
Savino, Alessandro
Schmidt, Manfred
Fronza, Raffaele
Common integration sites of published datasets identified using a graph-based framework
title Common integration sites of published datasets identified using a graph-based framework
title_full Common integration sites of published datasets identified using a graph-based framework
title_fullStr Common integration sites of published datasets identified using a graph-based framework
title_full_unstemmed Common integration sites of published datasets identified using a graph-based framework
title_short Common integration sites of published datasets identified using a graph-based framework
title_sort common integration sites of published datasets identified using a graph-based framework
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874583/
https://www.ncbi.nlm.nih.gov/pubmed/27257471
http://dx.doi.org/10.1016/j.csbj.2015.11.004
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