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Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization
Extraction of compounds from microalgae requires cell disruption as a pretreatment to increase extraction yield. Botryococcus braunii is a microalga with a significant content of carotenoids and other antioxidant compounds, such as chlorophylls. Cell disruption of B. braunii using CO(2) rapid depres...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874585/ https://www.ncbi.nlm.nih.gov/pubmed/26991281 http://dx.doi.org/10.1016/j.bjm.2016.01.020 |
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author | Uquiche, Edgar Antilaf, Ivette Millao, Sonia |
author_facet | Uquiche, Edgar Antilaf, Ivette Millao, Sonia |
author_sort | Uquiche, Edgar |
collection | PubMed |
description | Extraction of compounds from microalgae requires cell disruption as a pretreatment to increase extraction yield. Botryococcus braunii is a microalga with a significant content of carotenoids and other antioxidant compounds, such as chlorophylls. Cell disruption of B. braunii using CO(2) rapid depressurization was studied as a pretreatment for the extraction of carotenoid and chlorophyll pigments. We studied the effect of temperature (21–49 °C) and pressure (6–13 MPa) during static compression on pigment recovery with supercritical CO(2) at 40 °C, 30 MPa and solvent flow of 4.7 L NPT/min. Within the experimental region, the extraction yield of carotenoids and chlorophylls increased by 2.4- and 2.2-fold respectively. Static compression conditions of high pressure and low temperature increased the extraction of carotenoids and especially chlorophylls. We selected 21 °C and 13 MPa as the cell disruption condition, which produced 1.91 g/kg d.s. of carotenoids and 14.03 mg/kg d.s. of chlorophylls. Pretreated microalga gave a 10-fold higher chlorophyll extraction yield compared to the untreated sample. While for carotenoids and tocopherols were 1.25 and 1.14-fold higher, respectively. Additionally, antioxidant activity of pretreated microalga (33.22 mmol TE/kg oil) was significantly higher than the value for the untreated samples (29.11 mmol TE/kg oil) (p ≤ 0.05). Confocal microscopy images showed morphological differences between micro-colonies with and without disruption treatment, suggesting that partial cell disruption by rapid depressurization improved the extraction of microalga compounds. |
format | Online Article Text |
id | pubmed-4874585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48745852016-05-27 Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization Uquiche, Edgar Antilaf, Ivette Millao, Sonia Braz J Microbiol Industrial Microbiology Extraction of compounds from microalgae requires cell disruption as a pretreatment to increase extraction yield. Botryococcus braunii is a microalga with a significant content of carotenoids and other antioxidant compounds, such as chlorophylls. Cell disruption of B. braunii using CO(2) rapid depressurization was studied as a pretreatment for the extraction of carotenoid and chlorophyll pigments. We studied the effect of temperature (21–49 °C) and pressure (6–13 MPa) during static compression on pigment recovery with supercritical CO(2) at 40 °C, 30 MPa and solvent flow of 4.7 L NPT/min. Within the experimental region, the extraction yield of carotenoids and chlorophylls increased by 2.4- and 2.2-fold respectively. Static compression conditions of high pressure and low temperature increased the extraction of carotenoids and especially chlorophylls. We selected 21 °C and 13 MPa as the cell disruption condition, which produced 1.91 g/kg d.s. of carotenoids and 14.03 mg/kg d.s. of chlorophylls. Pretreated microalga gave a 10-fold higher chlorophyll extraction yield compared to the untreated sample. While for carotenoids and tocopherols were 1.25 and 1.14-fold higher, respectively. Additionally, antioxidant activity of pretreated microalga (33.22 mmol TE/kg oil) was significantly higher than the value for the untreated samples (29.11 mmol TE/kg oil) (p ≤ 0.05). Confocal microscopy images showed morphological differences between micro-colonies with and without disruption treatment, suggesting that partial cell disruption by rapid depressurization improved the extraction of microalga compounds. Elsevier 2016-03-02 /pmc/articles/PMC4874585/ /pubmed/26991281 http://dx.doi.org/10.1016/j.bjm.2016.01.020 Text en © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Industrial Microbiology Uquiche, Edgar Antilaf, Ivette Millao, Sonia Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization |
title | Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization |
title_full | Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization |
title_fullStr | Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization |
title_full_unstemmed | Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization |
title_short | Enhancement of pigment extraction from B. braunii pretreated using CO(2) rapid depressurization |
title_sort | enhancement of pigment extraction from b. braunii pretreated using co(2) rapid depressurization |
topic | Industrial Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874585/ https://www.ncbi.nlm.nih.gov/pubmed/26991281 http://dx.doi.org/10.1016/j.bjm.2016.01.020 |
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