Cargando…

High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media

Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient...

Descripción completa

Detalles Bibliográficos
Autores principales: Fiore, Antonio, Zhang, Jitao, Shao, Peng, Yun, Seok Hyun, Scarcelli, Giuliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874928/
https://www.ncbi.nlm.nih.gov/pubmed/27274097
http://dx.doi.org/10.1063/1.4948353
Descripción
Sumario:Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we combined a multi-pass Fabry-Perot interferometer with a two-stage virtually imaged phased array spectrometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100 μm deep within chicken muscle tissue.