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High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media

Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient...

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Autores principales: Fiore, Antonio, Zhang, Jitao, Shao, Peng, Yun, Seok Hyun, Scarcelli, Giuliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874928/
https://www.ncbi.nlm.nih.gov/pubmed/27274097
http://dx.doi.org/10.1063/1.4948353
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author Fiore, Antonio
Zhang, Jitao
Shao, Peng
Yun, Seok Hyun
Scarcelli, Giuliano
author_facet Fiore, Antonio
Zhang, Jitao
Shao, Peng
Yun, Seok Hyun
Scarcelli, Giuliano
author_sort Fiore, Antonio
collection PubMed
description Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we combined a multi-pass Fabry-Perot interferometer with a two-stage virtually imaged phased array spectrometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100 μm deep within chicken muscle tissue.
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spelling pubmed-48749282016-06-07 High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media Fiore, Antonio Zhang, Jitao Shao, Peng Yun, Seok Hyun Scarcelli, Giuliano Appl Phys Lett Biophysics and Bio-Inspired Systems Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we combined a multi-pass Fabry-Perot interferometer with a two-stage virtually imaged phased array spectrometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100 μm deep within chicken muscle tissue. AIP Publishing LLC 2016-05-16 2016-05-17 /pmc/articles/PMC4874928/ /pubmed/27274097 http://dx.doi.org/10.1063/1.4948353 Text en © 2016 Author(s). 0003-6951/2016/108(20)/203701/3 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biophysics and Bio-Inspired Systems
Fiore, Antonio
Zhang, Jitao
Shao, Peng
Yun, Seok Hyun
Scarcelli, Giuliano
High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
title High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
title_full High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
title_fullStr High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
title_full_unstemmed High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
title_short High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
title_sort high-extinction virtually imaged phased array-based brillouin spectroscopy of turbid biological media
topic Biophysics and Bio-Inspired Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874928/
https://www.ncbi.nlm.nih.gov/pubmed/27274097
http://dx.doi.org/10.1063/1.4948353
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