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High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media
Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874928/ https://www.ncbi.nlm.nih.gov/pubmed/27274097 http://dx.doi.org/10.1063/1.4948353 |
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author | Fiore, Antonio Zhang, Jitao Shao, Peng Yun, Seok Hyun Scarcelli, Giuliano |
author_facet | Fiore, Antonio Zhang, Jitao Shao, Peng Yun, Seok Hyun Scarcelli, Giuliano |
author_sort | Fiore, Antonio |
collection | PubMed |
description | Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we combined a multi-pass Fabry-Perot interferometer with a two-stage virtually imaged phased array spectrometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100 μm deep within chicken muscle tissue. |
format | Online Article Text |
id | pubmed-4874928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-48749282016-06-07 High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media Fiore, Antonio Zhang, Jitao Shao, Peng Yun, Seok Hyun Scarcelli, Giuliano Appl Phys Lett Biophysics and Bio-Inspired Systems Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we combined a multi-pass Fabry-Perot interferometer with a two-stage virtually imaged phased array spectrometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100 μm deep within chicken muscle tissue. AIP Publishing LLC 2016-05-16 2016-05-17 /pmc/articles/PMC4874928/ /pubmed/27274097 http://dx.doi.org/10.1063/1.4948353 Text en © 2016 Author(s). 0003-6951/2016/108(20)/203701/3 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Biophysics and Bio-Inspired Systems Fiore, Antonio Zhang, Jitao Shao, Peng Yun, Seok Hyun Scarcelli, Giuliano High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media |
title | High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media |
title_full | High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media |
title_fullStr | High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media |
title_full_unstemmed | High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media |
title_short | High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media |
title_sort | high-extinction virtually imaged phased array-based brillouin spectroscopy of turbid biological media |
topic | Biophysics and Bio-Inspired Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874928/ https://www.ncbi.nlm.nih.gov/pubmed/27274097 http://dx.doi.org/10.1063/1.4948353 |
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