Equilibria Between Cell Membranes and Electrolyte Solution: Effect of Fatal Accidental Hypothermia

Equilibria between the membranes of erythrocytes as well as thrombocytes and solution ions in fatal accidental hypothermia were analyzed using a theoretical four-equilibria model. The model was developed to determinate parameters characterizing cell membrane—surrounding ion interactions: the total surface concentrations of both acidic and basic groups C(A), C(B), and association constants K(AH), K(BOH). Knowledge of these parameters was necessary to calculate the theoretical values of surface charge density. The model was validated by curve-fitting the experimental data points to simulated data generated by the model. The experimental and theoretical surface charge density values agree at pH 2–8, at higher pH the deviation was observed.