Cargando…

The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study

Characterisation of the electrostatic properties of dental enamel is important for understanding the interfacial processes that occur on a tooth surface and how these relate to the natural ability of our teeth to withstand chemical attack from the acids in many soft drinks. Whereas, the role of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Lubarsky, Gennady V., D’Sa, Raechelle A., Deb, Sanjukta, Meenan, Brian J., Lemoine, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875143/
https://www.ncbi.nlm.nih.gov/pubmed/22589057
http://dx.doi.org/10.1007/s13758-011-0014-6
_version_ 1782433104710860800
author Lubarsky, Gennady V.
D’Sa, Raechelle A.
Deb, Sanjukta
Meenan, Brian J.
Lemoine, Patrick
author_facet Lubarsky, Gennady V.
D’Sa, Raechelle A.
Deb, Sanjukta
Meenan, Brian J.
Lemoine, Patrick
author_sort Lubarsky, Gennady V.
collection PubMed
description Characterisation of the electrostatic properties of dental enamel is important for understanding the interfacial processes that occur on a tooth surface and how these relate to the natural ability of our teeth to withstand chemical attack from the acids in many soft drinks. Whereas, the role of the mineral component of the tooth enamel in providing this resistance to acid erosion has been studied extensively, the influence of proteins that are also present within the structure is not well understood. In this paper, we report for the first time the use of double-layer force spectroscopy to directly measure electrostatic forces on as received and hydrazine-treated (deproteinated) enamel surfaces in solutions with different pH to determine how the enamel proteins influence acid erosion surface potential and surface charge of human dental enamel. The deproteination of the treated samples was confirmed by the loss of the amide bands (~1,300–1,700 cm(−1)) in the FTIR spectrum of the sample. The force characteristics observed were found to agree with the theory of electrical double layer interaction under the assumption of constant potential and allowed the surface charge per unit area to be determined for the two enamel surfaces. The values and, importantly, the sign of these adsorbed surface charges indicates that the protein content of dental enamel contributes significantly to the electrostatic double layer formation near the tooth surface and in doing so can buffer the apatite crystals against acid attack. Moreover, the electrostatic interactions within this layer are a driving factor for the mineral transfer from the tooth surface and the initial salivary pellicle formation.
format Online
Article
Text
id pubmed-4875143
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-48751432016-06-21 The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study Lubarsky, Gennady V. D’Sa, Raechelle A. Deb, Sanjukta Meenan, Brian J. Lemoine, Patrick Biointerphases Article Characterisation of the electrostatic properties of dental enamel is important for understanding the interfacial processes that occur on a tooth surface and how these relate to the natural ability of our teeth to withstand chemical attack from the acids in many soft drinks. Whereas, the role of the mineral component of the tooth enamel in providing this resistance to acid erosion has been studied extensively, the influence of proteins that are also present within the structure is not well understood. In this paper, we report for the first time the use of double-layer force spectroscopy to directly measure electrostatic forces on as received and hydrazine-treated (deproteinated) enamel surfaces in solutions with different pH to determine how the enamel proteins influence acid erosion surface potential and surface charge of human dental enamel. The deproteination of the treated samples was confirmed by the loss of the amide bands (~1,300–1,700 cm(−1)) in the FTIR spectrum of the sample. The force characteristics observed were found to agree with the theory of electrical double layer interaction under the assumption of constant potential and allowed the surface charge per unit area to be determined for the two enamel surfaces. The values and, importantly, the sign of these adsorbed surface charges indicates that the protein content of dental enamel contributes significantly to the electrostatic double layer formation near the tooth surface and in doing so can buffer the apatite crystals against acid attack. Moreover, the electrostatic interactions within this layer are a driving factor for the mineral transfer from the tooth surface and the initial salivary pellicle formation. Springer Berlin Heidelberg 2012-02-17 /pmc/articles/PMC4875143/ /pubmed/22589057 http://dx.doi.org/10.1007/s13758-011-0014-6 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Lubarsky, Gennady V.
D’Sa, Raechelle A.
Deb, Sanjukta
Meenan, Brian J.
Lemoine, Patrick
The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study
title The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study
title_full The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study
title_fullStr The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study
title_full_unstemmed The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study
title_short The Role of Enamel Proteins in Protecting Mature Human Enamel Against Acidic Environments: A Double Layer Force Spectroscopy Study
title_sort role of enamel proteins in protecting mature human enamel against acidic environments: a double layer force spectroscopy study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875143/
https://www.ncbi.nlm.nih.gov/pubmed/22589057
http://dx.doi.org/10.1007/s13758-011-0014-6
work_keys_str_mv AT lubarskygennadyv theroleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT dsaraechellea theroleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT debsanjukta theroleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT meenanbrianj theroleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT lemoinepatrick theroleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT lubarskygennadyv roleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT dsaraechellea roleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT debsanjukta roleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT meenanbrianj roleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy
AT lemoinepatrick roleofenamelproteinsinprotectingmaturehumanenamelagainstacidicenvironmentsadoublelayerforcespectroscopystudy