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Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1

Members of the genus Leptospirillum are aerobic iron-oxidizing bacteria belonging to the phylum Nitrospira. They are important members of microbial communities that catalyze the biomining of sulfidic ores, thereby solubilizing metal ions. These microorganisms live under extremely acidic and metal-lo...

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Autores principales: Ferrer, Alonso, Rivera, Javier, Zapata, Claudia, Norambuena, Javiera, Sandoval, Álvaro, Chávez, Renato, Orellana, Omar, Levicán, Gloria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876134/
https://www.ncbi.nlm.nih.gov/pubmed/27242761
http://dx.doi.org/10.3389/fmicb.2016.00748
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author Ferrer, Alonso
Rivera, Javier
Zapata, Claudia
Norambuena, Javiera
Sandoval, Álvaro
Chávez, Renato
Orellana, Omar
Levicán, Gloria
author_facet Ferrer, Alonso
Rivera, Javier
Zapata, Claudia
Norambuena, Javiera
Sandoval, Álvaro
Chávez, Renato
Orellana, Omar
Levicán, Gloria
author_sort Ferrer, Alonso
collection PubMed
description Members of the genus Leptospirillum are aerobic iron-oxidizing bacteria belonging to the phylum Nitrospira. They are important members of microbial communities that catalyze the biomining of sulfidic ores, thereby solubilizing metal ions. These microorganisms live under extremely acidic and metal-loaded environments and thus must tolerate high concentrations of reactive oxygen species (ROS). Cobalamin (vitamin B(12)) is a cobalt-containing tetrapyrrole cofactor involved in intramolecular rearrangement reactions and has recently been suggested to be an intracellular antioxidant. In this work, we investigated the effect of the exogenous addition of cobalamin on oxidative stress parameters in Leptospirillum group II strain CF-1. Our results revealed that the external supplementation of cobalamin reduces the levels of intracellular ROSs and the damage to biomolecules, and also stimulates the growth and survival of cells exposed to oxidative stress exerted by ferric ion, hydrogen peroxide, chromate and diamide. Furthermore, exposure of strain CF-1 to oxidative stress elicitors resulted in the transcriptional activation of the cbiA gene encoding CbiA of the cobalamin biosynthetic pathway. Altogether, these data suggest that cobalamin plays an important role in redox protection of Leptospirillum strain CF-1, supporting survival of this microorganism under extremely oxidative environmental conditions. Understanding the mechanisms underlying the protective effect of cobalamin against oxidative stress may help to develop strategies to make biomining processes more effective.
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spelling pubmed-48761342016-05-30 Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1 Ferrer, Alonso Rivera, Javier Zapata, Claudia Norambuena, Javiera Sandoval, Álvaro Chávez, Renato Orellana, Omar Levicán, Gloria Front Microbiol Microbiology Members of the genus Leptospirillum are aerobic iron-oxidizing bacteria belonging to the phylum Nitrospira. They are important members of microbial communities that catalyze the biomining of sulfidic ores, thereby solubilizing metal ions. These microorganisms live under extremely acidic and metal-loaded environments and thus must tolerate high concentrations of reactive oxygen species (ROS). Cobalamin (vitamin B(12)) is a cobalt-containing tetrapyrrole cofactor involved in intramolecular rearrangement reactions and has recently been suggested to be an intracellular antioxidant. In this work, we investigated the effect of the exogenous addition of cobalamin on oxidative stress parameters in Leptospirillum group II strain CF-1. Our results revealed that the external supplementation of cobalamin reduces the levels of intracellular ROSs and the damage to biomolecules, and also stimulates the growth and survival of cells exposed to oxidative stress exerted by ferric ion, hydrogen peroxide, chromate and diamide. Furthermore, exposure of strain CF-1 to oxidative stress elicitors resulted in the transcriptional activation of the cbiA gene encoding CbiA of the cobalamin biosynthetic pathway. Altogether, these data suggest that cobalamin plays an important role in redox protection of Leptospirillum strain CF-1, supporting survival of this microorganism under extremely oxidative environmental conditions. Understanding the mechanisms underlying the protective effect of cobalamin against oxidative stress may help to develop strategies to make biomining processes more effective. Frontiers Media S.A. 2016-05-23 /pmc/articles/PMC4876134/ /pubmed/27242761 http://dx.doi.org/10.3389/fmicb.2016.00748 Text en Copyright © 2016 Ferrer, Rivera, Zapata, Norambuena, Sandoval, Chávez, Orellana and Levicán. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ferrer, Alonso
Rivera, Javier
Zapata, Claudia
Norambuena, Javiera
Sandoval, Álvaro
Chávez, Renato
Orellana, Omar
Levicán, Gloria
Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1
title Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1
title_full Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1
title_fullStr Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1
title_full_unstemmed Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1
title_short Cobalamin Protection against Oxidative Stress in the Acidophilic Iron-oxidizing Bacterium Leptospirillum Group II CF-1
title_sort cobalamin protection against oxidative stress in the acidophilic iron-oxidizing bacterium leptospirillum group ii cf-1
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876134/
https://www.ncbi.nlm.nih.gov/pubmed/27242761
http://dx.doi.org/10.3389/fmicb.2016.00748
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