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Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death

BACKGROUND: Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their...

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Autores principales: Tan, Shaun S., Han, Xiaolian, Sivakumaran, Priyadharshini, Lim, Shiang Y., Morrison, Wayne A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Plastic and Reconstructive Surgeons 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876151/
https://www.ncbi.nlm.nih.gov/pubmed/27218020
http://dx.doi.org/10.5999/aps.2016.43.3.237
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author Tan, Shaun S.
Han, Xiaolian
Sivakumaran, Priyadharshini
Lim, Shiang Y.
Morrison, Wayne A.
author_facet Tan, Shaun S.
Han, Xiaolian
Sivakumaran, Priyadharshini
Lim, Shiang Y.
Morrison, Wayne A.
author_sort Tan, Shaun S.
collection PubMed
description BACKGROUND: Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs. METHODS: We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours. Cell viability and apoptosis were determined with propidium iodide and Hoechst 33342 staining under fluorescence microscopy. RESULTS: Hydrogen peroxide (1–2.5 mM) treatment resulted in an incremental increase in cell death. 2 mM hydrogen peroxide was thereafter selected as the dose for co-treatment with melatonin. Melatonin alone had no adverse effects on ASCs. Co-treatment of ASCs with melatonin in the presence of hydrogen peroxide protected ASCs from cell death in a dose-dependent manner, and afforded maximal protection at 100 µM (n=4, one-way analysis of variance P<0.001). Melatonin co-treated ASCs displayed significantly fewer apoptotic cells, as demonstrated by condensed and fragmented nuclei under fluorescence microscopy. CONCLUSIONS: Melatonin possesses cytoprotective properties against oxidative stress in human ASCs and might be a useful adjunct in fat grafting and cell-assisted lipotransfer.
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spelling pubmed-48761512016-05-23 Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death Tan, Shaun S. Han, Xiaolian Sivakumaran, Priyadharshini Lim, Shiang Y. Morrison, Wayne A. Arch Plast Surg Original Article BACKGROUND: Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs. METHODS: We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours. Cell viability and apoptosis were determined with propidium iodide and Hoechst 33342 staining under fluorescence microscopy. RESULTS: Hydrogen peroxide (1–2.5 mM) treatment resulted in an incremental increase in cell death. 2 mM hydrogen peroxide was thereafter selected as the dose for co-treatment with melatonin. Melatonin alone had no adverse effects on ASCs. Co-treatment of ASCs with melatonin in the presence of hydrogen peroxide protected ASCs from cell death in a dose-dependent manner, and afforded maximal protection at 100 µM (n=4, one-way analysis of variance P<0.001). Melatonin co-treated ASCs displayed significantly fewer apoptotic cells, as demonstrated by condensed and fragmented nuclei under fluorescence microscopy. CONCLUSIONS: Melatonin possesses cytoprotective properties against oxidative stress in human ASCs and might be a useful adjunct in fat grafting and cell-assisted lipotransfer. The Korean Society of Plastic and Reconstructive Surgeons 2016-05 2016-05-18 /pmc/articles/PMC4876151/ /pubmed/27218020 http://dx.doi.org/10.5999/aps.2016.43.3.237 Text en Copyright © 2016 The Korean Society of Plastic and Reconstructive Surgeons http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tan, Shaun S.
Han, Xiaolian
Sivakumaran, Priyadharshini
Lim, Shiang Y.
Morrison, Wayne A.
Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death
title Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death
title_full Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death
title_fullStr Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death
title_full_unstemmed Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death
title_short Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death
title_sort melatonin protects human adipose-derived stem cells from oxidative stress and cell death
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876151/
https://www.ncbi.nlm.nih.gov/pubmed/27218020
http://dx.doi.org/10.5999/aps.2016.43.3.237
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