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Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device
Background. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stack...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876222/ https://www.ncbi.nlm.nih.gov/pubmed/27247943 http://dx.doi.org/10.1155/2016/6939018 |
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author | Manuskiatti, Woraphong Pattanaprichakul, Penvadee Inthasotti, Siriluk Sitthinamsuwan, Panitta Hanamornroongruang, Suchanan Wanitphakdeedecha, Rungsima Chu-ongsakol, Sorawuth |
author_facet | Manuskiatti, Woraphong Pattanaprichakul, Penvadee Inthasotti, Siriluk Sitthinamsuwan, Panitta Hanamornroongruang, Suchanan Wanitphakdeedecha, Rungsima Chu-ongsakol, Sorawuth |
author_sort | Manuskiatti, Woraphong |
collection | PubMed |
description | Background. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stacking of a FRMS in dark-skinned subjects. Methods. Two female volunteers who were scheduled for abdominoplasty received treatment with a FRMS with varying energy settings at 6 time periods including 3 months, 1 month, 1 week, 3 days, 1 day, and the time immediately before abdominoplasty. Biopsy specimens were analyzed using hematoxylin and eosin (H&E), Verhoeff-Van Gieson (VVG), colloidal iron, and Fontana-Masson stain. Immunohistochemical study was performed by using Heat Shock Protein 70 (HSP70) antibody and collagen III monoclonal antibody. Results. The average depth of radiofrequency thermal zone (RFTZ) ranged from 100 to 300 μm, correlating with energy levels. Columns of cell necrosis and collagen denaturation followed by inflammatory response were initially demonstrated, with subsequent increasing of mucin at 1 and 3 months after treatment. Immunohistochemical study showed positive stain with HSP70. Conclusion. A single treatment with a FRMS using appropriate energy setting induces neocollagenesis. This wound healing response may serve as a mean to improve the appearance of photodamaged skin and atrophic scars. |
format | Online Article Text |
id | pubmed-4876222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48762222016-05-31 Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device Manuskiatti, Woraphong Pattanaprichakul, Penvadee Inthasotti, Siriluk Sitthinamsuwan, Panitta Hanamornroongruang, Suchanan Wanitphakdeedecha, Rungsima Chu-ongsakol, Sorawuth Biomed Res Int Research Article Background. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stacking of a FRMS in dark-skinned subjects. Methods. Two female volunteers who were scheduled for abdominoplasty received treatment with a FRMS with varying energy settings at 6 time periods including 3 months, 1 month, 1 week, 3 days, 1 day, and the time immediately before abdominoplasty. Biopsy specimens were analyzed using hematoxylin and eosin (H&E), Verhoeff-Van Gieson (VVG), colloidal iron, and Fontana-Masson stain. Immunohistochemical study was performed by using Heat Shock Protein 70 (HSP70) antibody and collagen III monoclonal antibody. Results. The average depth of radiofrequency thermal zone (RFTZ) ranged from 100 to 300 μm, correlating with energy levels. Columns of cell necrosis and collagen denaturation followed by inflammatory response were initially demonstrated, with subsequent increasing of mucin at 1 and 3 months after treatment. Immunohistochemical study showed positive stain with HSP70. Conclusion. A single treatment with a FRMS using appropriate energy setting induces neocollagenesis. This wound healing response may serve as a mean to improve the appearance of photodamaged skin and atrophic scars. Hindawi Publishing Corporation 2016 2016-05-09 /pmc/articles/PMC4876222/ /pubmed/27247943 http://dx.doi.org/10.1155/2016/6939018 Text en Copyright © 2016 Woraphong Manuskiatti et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Manuskiatti, Woraphong Pattanaprichakul, Penvadee Inthasotti, Siriluk Sitthinamsuwan, Panitta Hanamornroongruang, Suchanan Wanitphakdeedecha, Rungsima Chu-ongsakol, Sorawuth Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title | Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_full | Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_fullStr | Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_full_unstemmed | Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_short | Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_sort | thermal response of in vivo human skin to fractional radiofrequency microneedle device |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876222/ https://www.ncbi.nlm.nih.gov/pubmed/27247943 http://dx.doi.org/10.1155/2016/6939018 |
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