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Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene

Respiratory Syncytial Virus (RSV) is responsible for considerable morbidity and mortality worldwide and is the most important respiratory viral pathogen in infants. Extensive sequence variability within and between RSV group A and B viruses and the ability of multiple clades and sub-clades of RSV to...

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Autores principales: Schobel, Seth A., Stucker, Karla M., Moore, Martin L., Anderson, Larry J., Larkin, Emma K., Shankar, Jyoti, Bera, Jayati, Puri, Vinita, Shilts, Meghan H., Rosas-Salazar, Christian, Halpin, Rebecca A., Fedorova, Nadia, Shrivastava, Susmita, Stockwell, Timothy B., Peebles, R. Stokes, Hartert, Tina V., Das, Suman R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876326/
https://www.ncbi.nlm.nih.gov/pubmed/27212633
http://dx.doi.org/10.1038/srep26311
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author Schobel, Seth A.
Stucker, Karla M.
Moore, Martin L.
Anderson, Larry J.
Larkin, Emma K.
Shankar, Jyoti
Bera, Jayati
Puri, Vinita
Shilts, Meghan H.
Rosas-Salazar, Christian
Halpin, Rebecca A.
Fedorova, Nadia
Shrivastava, Susmita
Stockwell, Timothy B.
Peebles, R. Stokes
Hartert, Tina V.
Das, Suman R.
author_facet Schobel, Seth A.
Stucker, Karla M.
Moore, Martin L.
Anderson, Larry J.
Larkin, Emma K.
Shankar, Jyoti
Bera, Jayati
Puri, Vinita
Shilts, Meghan H.
Rosas-Salazar, Christian
Halpin, Rebecca A.
Fedorova, Nadia
Shrivastava, Susmita
Stockwell, Timothy B.
Peebles, R. Stokes
Hartert, Tina V.
Das, Suman R.
author_sort Schobel, Seth A.
collection PubMed
description Respiratory Syncytial Virus (RSV) is responsible for considerable morbidity and mortality worldwide and is the most important respiratory viral pathogen in infants. Extensive sequence variability within and between RSV group A and B viruses and the ability of multiple clades and sub-clades of RSV to co-circulate are likely mechanisms contributing to the evasion of herd immunity. Surveillance and large-scale whole-genome sequencing of RSV is currently limited but would help identify its evolutionary dynamics and sites of selective immune evasion. In this study, we performed complete-genome next-generation sequencing of 92 RSV isolates from infants in central Tennessee during the 2012–2014 RSV seasons. We identified multiple co-circulating clades of RSV from both the A and B groups. Each clade is defined by signature N- and O-linked glycosylation patterns. Analyses of specific RSV genes revealed high rates of positive selection in the attachment (G) gene. We identified RSV-A viruses in circulation with and without a recently reported 72-nucleotide G gene sequence duplication. Furthermore, we show evidence of convergent evolution of G gene sequence duplication and fixation over time, which suggests a potential fitness advantage of RSV with the G sequence duplication.
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spelling pubmed-48763262016-06-06 Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene Schobel, Seth A. Stucker, Karla M. Moore, Martin L. Anderson, Larry J. Larkin, Emma K. Shankar, Jyoti Bera, Jayati Puri, Vinita Shilts, Meghan H. Rosas-Salazar, Christian Halpin, Rebecca A. Fedorova, Nadia Shrivastava, Susmita Stockwell, Timothy B. Peebles, R. Stokes Hartert, Tina V. Das, Suman R. Sci Rep Article Respiratory Syncytial Virus (RSV) is responsible for considerable morbidity and mortality worldwide and is the most important respiratory viral pathogen in infants. Extensive sequence variability within and between RSV group A and B viruses and the ability of multiple clades and sub-clades of RSV to co-circulate are likely mechanisms contributing to the evasion of herd immunity. Surveillance and large-scale whole-genome sequencing of RSV is currently limited but would help identify its evolutionary dynamics and sites of selective immune evasion. In this study, we performed complete-genome next-generation sequencing of 92 RSV isolates from infants in central Tennessee during the 2012–2014 RSV seasons. We identified multiple co-circulating clades of RSV from both the A and B groups. Each clade is defined by signature N- and O-linked glycosylation patterns. Analyses of specific RSV genes revealed high rates of positive selection in the attachment (G) gene. We identified RSV-A viruses in circulation with and without a recently reported 72-nucleotide G gene sequence duplication. Furthermore, we show evidence of convergent evolution of G gene sequence duplication and fixation over time, which suggests a potential fitness advantage of RSV with the G sequence duplication. Nature Publishing Group 2016-05-23 /pmc/articles/PMC4876326/ /pubmed/27212633 http://dx.doi.org/10.1038/srep26311 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Schobel, Seth A.
Stucker, Karla M.
Moore, Martin L.
Anderson, Larry J.
Larkin, Emma K.
Shankar, Jyoti
Bera, Jayati
Puri, Vinita
Shilts, Meghan H.
Rosas-Salazar, Christian
Halpin, Rebecca A.
Fedorova, Nadia
Shrivastava, Susmita
Stockwell, Timothy B.
Peebles, R. Stokes
Hartert, Tina V.
Das, Suman R.
Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene
title Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene
title_full Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene
title_fullStr Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene
title_full_unstemmed Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene
title_short Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene
title_sort respiratory syncytial virus whole-genome sequencing identifies convergent evolution of sequence duplication in the c-terminus of the g gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876326/
https://www.ncbi.nlm.nih.gov/pubmed/27212633
http://dx.doi.org/10.1038/srep26311
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