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Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis

Apical-basal polarity plays critical roles in the functions of epithelial tissues. However, the mechanisms of epithelial polarity establishment and maintenance remain to be fully elucidated. Here we show that the membrane-associated guanylate kinase (MAGUK) family protein Dlg5 is required for the ma...

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Autores principales: Luo, Jun, Wang, Heng, Kang, Di, Guo, Xuan, Wan, Ping, Wang, Dou, Chen, Jiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876392/
https://www.ncbi.nlm.nih.gov/pubmed/27211898
http://dx.doi.org/10.1038/srep26553
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author Luo, Jun
Wang, Heng
Kang, Di
Guo, Xuan
Wan, Ping
Wang, Dou
Chen, Jiong
author_facet Luo, Jun
Wang, Heng
Kang, Di
Guo, Xuan
Wan, Ping
Wang, Dou
Chen, Jiong
author_sort Luo, Jun
collection PubMed
description Apical-basal polarity plays critical roles in the functions of epithelial tissues. However, the mechanisms of epithelial polarity establishment and maintenance remain to be fully elucidated. Here we show that the membrane-associated guanylate kinase (MAGUK) family protein Dlg5 is required for the maintenance of apical polarity of follicle epithelium during Drosophila oogenesis. Dlg5 localizes at the apical membrane and adherens junction (AJ) of follicle epithelium in early stage egg chambers. Specifically, we demonstrate that the major function of Dlg5 is to promote apical membrane localization of Crumbs, since overexpression of Crumbs but not other major apical or AJ components could rescue epithelial polarity defects resulted from loss of Dlg5. Furthermore, we performed a structure-function analysis of Dlg5 and found that the C-terminal PDZ3 and PDZ4 domains are required for all Dlg5’s functions as well as its ability to localize to apical membrane. The N-terminal coiled-coil motif could be individually targeted to the apical membrane, while the central linker region could be targeted to AJ. Lastly, the MAGUK core domains of PDZ4-SH3-GUK could be individually targeted to apical, AJ and basolateral membranes.
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spelling pubmed-48763922016-06-06 Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis Luo, Jun Wang, Heng Kang, Di Guo, Xuan Wan, Ping Wang, Dou Chen, Jiong Sci Rep Article Apical-basal polarity plays critical roles in the functions of epithelial tissues. However, the mechanisms of epithelial polarity establishment and maintenance remain to be fully elucidated. Here we show that the membrane-associated guanylate kinase (MAGUK) family protein Dlg5 is required for the maintenance of apical polarity of follicle epithelium during Drosophila oogenesis. Dlg5 localizes at the apical membrane and adherens junction (AJ) of follicle epithelium in early stage egg chambers. Specifically, we demonstrate that the major function of Dlg5 is to promote apical membrane localization of Crumbs, since overexpression of Crumbs but not other major apical or AJ components could rescue epithelial polarity defects resulted from loss of Dlg5. Furthermore, we performed a structure-function analysis of Dlg5 and found that the C-terminal PDZ3 and PDZ4 domains are required for all Dlg5’s functions as well as its ability to localize to apical membrane. The N-terminal coiled-coil motif could be individually targeted to the apical membrane, while the central linker region could be targeted to AJ. Lastly, the MAGUK core domains of PDZ4-SH3-GUK could be individually targeted to apical, AJ and basolateral membranes. Nature Publishing Group 2016-05-23 /pmc/articles/PMC4876392/ /pubmed/27211898 http://dx.doi.org/10.1038/srep26553 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Luo, Jun
Wang, Heng
Kang, Di
Guo, Xuan
Wan, Ping
Wang, Dou
Chen, Jiong
Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis
title Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis
title_full Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis
title_fullStr Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis
title_full_unstemmed Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis
title_short Dlg5 maintains apical polarity by promoting membrane localization of Crumbs during Drosophila oogenesis
title_sort dlg5 maintains apical polarity by promoting membrane localization of crumbs during drosophila oogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876392/
https://www.ncbi.nlm.nih.gov/pubmed/27211898
http://dx.doi.org/10.1038/srep26553
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