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Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice

Long-term, high dosage protamine zinc insulin (PZI) treatments produce adverse reactions. The trace element selenium (Se) is a candidate for the prevention of diabetes due to anti-oxidative stress activity and the regulation of glycometabolism. In this study, we aimed to investigate the anti-diabeti...

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Autores principales: Lu, Juan, Ji, Wenjun, Zhao, Mei, Wang, Meng, Yan, Wenhui, Chen, Mingxia, Ren, Shuting, Yuan, Bingxiang, Wang, Bing, Chen, Lina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876423/
https://www.ncbi.nlm.nih.gov/pubmed/27212152
http://dx.doi.org/10.1038/srep26563
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author Lu, Juan
Ji, Wenjun
Zhao, Mei
Wang, Meng
Yan, Wenhui
Chen, Mingxia
Ren, Shuting
Yuan, Bingxiang
Wang, Bing
Chen, Lina
author_facet Lu, Juan
Ji, Wenjun
Zhao, Mei
Wang, Meng
Yan, Wenhui
Chen, Mingxia
Ren, Shuting
Yuan, Bingxiang
Wang, Bing
Chen, Lina
author_sort Lu, Juan
collection PubMed
description Long-term, high dosage protamine zinc insulin (PZI) treatments produce adverse reactions. The trace element selenium (Se) is a candidate for the prevention of diabetes due to anti-oxidative stress activity and the regulation of glycometabolism. In this study, we aimed to investigate the anti-diabetic effects of a combination of PZI and Se on type 2 diabetes. Diabetic KKAy mice were randomized into the following groups: model group and groups that were subcutaneously injected with PZI, Se, high or low dose PZI + Se for 6 weeks. PZI combined with Se decreased the body weight and fasting blood glucose levels. Moreover, this treatment also improved insulin tolerance, as determined by the reduced values from the oral glucose tolerance test and insulin tolerance test, and increased insulin levels and insulin sensitivity index. PZI combined with Se ameliorated skeletal muscle and β-cell damage and the impaired mitochondrial morphology. Oxidative stress was also reduced. Furthermore, PZI combined with Se upregulated phosphatidylinositol 3-kinase (PI3K) and downregulated protein tyrosine phosphatase 1B (PTP1B). Importantly, the low dosage combination produced effects similar to PZI alone. In conclusion, PZI combined with Se improved glycometabolism and ameliorated the tissue and mitochondrial damage, which might be associated with the PI3K and PTP1B pathways.
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spelling pubmed-48764232016-06-06 Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice Lu, Juan Ji, Wenjun Zhao, Mei Wang, Meng Yan, Wenhui Chen, Mingxia Ren, Shuting Yuan, Bingxiang Wang, Bing Chen, Lina Sci Rep Article Long-term, high dosage protamine zinc insulin (PZI) treatments produce adverse reactions. The trace element selenium (Se) is a candidate for the prevention of diabetes due to anti-oxidative stress activity and the regulation of glycometabolism. In this study, we aimed to investigate the anti-diabetic effects of a combination of PZI and Se on type 2 diabetes. Diabetic KKAy mice were randomized into the following groups: model group and groups that were subcutaneously injected with PZI, Se, high or low dose PZI + Se for 6 weeks. PZI combined with Se decreased the body weight and fasting blood glucose levels. Moreover, this treatment also improved insulin tolerance, as determined by the reduced values from the oral glucose tolerance test and insulin tolerance test, and increased insulin levels and insulin sensitivity index. PZI combined with Se ameliorated skeletal muscle and β-cell damage and the impaired mitochondrial morphology. Oxidative stress was also reduced. Furthermore, PZI combined with Se upregulated phosphatidylinositol 3-kinase (PI3K) and downregulated protein tyrosine phosphatase 1B (PTP1B). Importantly, the low dosage combination produced effects similar to PZI alone. In conclusion, PZI combined with Se improved glycometabolism and ameliorated the tissue and mitochondrial damage, which might be associated with the PI3K and PTP1B pathways. Nature Publishing Group 2016-05-23 /pmc/articles/PMC4876423/ /pubmed/27212152 http://dx.doi.org/10.1038/srep26563 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lu, Juan
Ji, Wenjun
Zhao, Mei
Wang, Meng
Yan, Wenhui
Chen, Mingxia
Ren, Shuting
Yuan, Bingxiang
Wang, Bing
Chen, Lina
Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice
title Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice
title_full Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice
title_fullStr Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice
title_full_unstemmed Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice
title_short Protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic KKAy mice
title_sort protamine zinc insulin combined with sodium selenite improves glycometabolism in the diabetic kkay mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876423/
https://www.ncbi.nlm.nih.gov/pubmed/27212152
http://dx.doi.org/10.1038/srep26563
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