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Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor
Alternative splicing generates protein diversity essential for neuronal properties. However, the precise mechanisms underlying this process and its relevance to physiological and behavioural functions are poorly understood. To address these issues, we focused on a cassette exon of the Caenorhabditis...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876481/ https://www.ncbi.nlm.nih.gov/pubmed/27198602 http://dx.doi.org/10.1038/ncomms11645 |
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author | Tomioka, Masahiro Naito, Yasuki Kuroyanagi, Hidehito Iino, Yuichi |
author_facet | Tomioka, Masahiro Naito, Yasuki Kuroyanagi, Hidehito Iino, Yuichi |
author_sort | Tomioka, Masahiro |
collection | PubMed |
description | Alternative splicing generates protein diversity essential for neuronal properties. However, the precise mechanisms underlying this process and its relevance to physiological and behavioural functions are poorly understood. To address these issues, we focused on a cassette exon of the Caenorhabditis elegans insulin receptor gene daf-2, whose proper variant expression in the taste receptor neuron ASER is critical for taste-avoidance learning. We show that inclusion of daf-2 exon 11.5 is restricted to specific neuron types, including ASER, and is controlled by a combinatorial action of evolutionarily conserved alternative splicing factors, RBFOX, CELF and PTB families of proteins. Mutations of these factors cause a learning defect, and this defect is relieved by DAF-2c (exon 11.5+) isoform expression only in a single neuron ASER. Our results provide evidence that alternative splicing regulation of a single critical gene in a single critical neuron is essential for learning ability in an organism. |
format | Online Article Text |
id | pubmed-4876481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48764812016-06-02 Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor Tomioka, Masahiro Naito, Yasuki Kuroyanagi, Hidehito Iino, Yuichi Nat Commun Article Alternative splicing generates protein diversity essential for neuronal properties. However, the precise mechanisms underlying this process and its relevance to physiological and behavioural functions are poorly understood. To address these issues, we focused on a cassette exon of the Caenorhabditis elegans insulin receptor gene daf-2, whose proper variant expression in the taste receptor neuron ASER is critical for taste-avoidance learning. We show that inclusion of daf-2 exon 11.5 is restricted to specific neuron types, including ASER, and is controlled by a combinatorial action of evolutionarily conserved alternative splicing factors, RBFOX, CELF and PTB families of proteins. Mutations of these factors cause a learning defect, and this defect is relieved by DAF-2c (exon 11.5+) isoform expression only in a single neuron ASER. Our results provide evidence that alternative splicing regulation of a single critical gene in a single critical neuron is essential for learning ability in an organism. Nature Publishing Group 2016-05-20 /pmc/articles/PMC4876481/ /pubmed/27198602 http://dx.doi.org/10.1038/ncomms11645 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tomioka, Masahiro Naito, Yasuki Kuroyanagi, Hidehito Iino, Yuichi Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor |
title | Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor |
title_full | Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor |
title_fullStr | Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor |
title_full_unstemmed | Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor |
title_short | Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor |
title_sort | splicing factors control c. elegans behavioural learning in a single neuron by producing daf-2c receptor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876481/ https://www.ncbi.nlm.nih.gov/pubmed/27198602 http://dx.doi.org/10.1038/ncomms11645 |
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