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miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140

Members of the microRNA (miR)-30 family have been reported to promote adipogenesis and inhibit osteogenesis, yet their role in the regulation of thermogenesis remains unknown. In this study, we show that miR-30b/c concentrations are greatly increased during adipocyte differentiation and are stimulat...

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Autores principales: Hu, Fang, Wang, Min, Xiao, Ting, Yin, Bangqi, He, Linyun, Meng, Wen, Dong, Meijuan, Liu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Diabetes Association 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876748/
https://www.ncbi.nlm.nih.gov/pubmed/25576051
http://dx.doi.org/10.2337/db14-1117
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author Hu, Fang
Wang, Min
Xiao, Ting
Yin, Bangqi
He, Linyun
Meng, Wen
Dong, Meijuan
Liu, Feng
author_facet Hu, Fang
Wang, Min
Xiao, Ting
Yin, Bangqi
He, Linyun
Meng, Wen
Dong, Meijuan
Liu, Feng
author_sort Hu, Fang
collection PubMed
description Members of the microRNA (miR)-30 family have been reported to promote adipogenesis and inhibit osteogenesis, yet their role in the regulation of thermogenesis remains unknown. In this study, we show that miR-30b/c concentrations are greatly increased during adipocyte differentiation and are stimulated by cold exposure or the β-adrenergic receptor activator. Overexpression and knockdown of miR-30b and -30c induced and suppressed, respectively, the expression of thermogenic genes such as UCP1 and Cidea in brown adipocytes. Forced expression of miR-30b/c also significantly increased thermogenic gene expression and mitochondrial respiration in primary adipocytes derived from subcutaneous white adipose tissue, demonstrating a promoting effect of miRNAs on the development of beige fat. In addition, knockdown of miR-30b/c repressed UCP1 expression in brown adipose tissue in vivo. miR-30b/c targets the 3′-untranslated region of the receptor-interacting protein 140 (RIP140), and overexpression of miR-30b/c significantly reduced RIP140 expression. Consistent with RIP140 as a target of miR-30b/c in regulating thermogenic gene expression, overexpression of RIP140 greatly suppressed the promoting effect of miR-30b/c on the expression of UCP1 and Cidea in brown adipocytes. Taken together, the data from our study identify miR-30b/c as a key regulator of thermogenesis and uncover a new mechanism underlying the regulation of brown adipose tissue function and the development of beige fat.
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spelling pubmed-48767482016-06-03 miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140 Hu, Fang Wang, Min Xiao, Ting Yin, Bangqi He, Linyun Meng, Wen Dong, Meijuan Liu, Feng Diabetes Signal Transduction Members of the microRNA (miR)-30 family have been reported to promote adipogenesis and inhibit osteogenesis, yet their role in the regulation of thermogenesis remains unknown. In this study, we show that miR-30b/c concentrations are greatly increased during adipocyte differentiation and are stimulated by cold exposure or the β-adrenergic receptor activator. Overexpression and knockdown of miR-30b and -30c induced and suppressed, respectively, the expression of thermogenic genes such as UCP1 and Cidea in brown adipocytes. Forced expression of miR-30b/c also significantly increased thermogenic gene expression and mitochondrial respiration in primary adipocytes derived from subcutaneous white adipose tissue, demonstrating a promoting effect of miRNAs on the development of beige fat. In addition, knockdown of miR-30b/c repressed UCP1 expression in brown adipose tissue in vivo. miR-30b/c targets the 3′-untranslated region of the receptor-interacting protein 140 (RIP140), and overexpression of miR-30b/c significantly reduced RIP140 expression. Consistent with RIP140 as a target of miR-30b/c in regulating thermogenic gene expression, overexpression of RIP140 greatly suppressed the promoting effect of miR-30b/c on the expression of UCP1 and Cidea in brown adipocytes. Taken together, the data from our study identify miR-30b/c as a key regulator of thermogenesis and uncover a new mechanism underlying the regulation of brown adipose tissue function and the development of beige fat. American Diabetes Association 2015-06 2015-01-09 /pmc/articles/PMC4876748/ /pubmed/25576051 http://dx.doi.org/10.2337/db14-1117 Text en © 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
spellingShingle Signal Transduction
Hu, Fang
Wang, Min
Xiao, Ting
Yin, Bangqi
He, Linyun
Meng, Wen
Dong, Meijuan
Liu, Feng
miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140
title miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140
title_full miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140
title_fullStr miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140
title_full_unstemmed miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140
title_short miR-30 Promotes Thermogenesis and the Development of Beige Fat by Targeting RIP140
title_sort mir-30 promotes thermogenesis and the development of beige fat by targeting rip140
topic Signal Transduction
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876748/
https://www.ncbi.nlm.nih.gov/pubmed/25576051
http://dx.doi.org/10.2337/db14-1117
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