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Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing

Selection of the optimal chemotherapy regimen for an individual cancer patient is challenging. The existing chemosensitivity tests are costly, time-consuming, and not amenable to wide utilization within a clinic. This limitation might be addressed by the recently proposed use of circulating tumor ce...

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Autores principales: Shen, Qinglin, Peng, Caixia, Zhan, Yan, Fan, Liang, Wang, Mengyi, Zhou, Qing, Liu, Jue, Lv, Xiaojuan, Tang, Qiu, Li, Jun, Huang, Xiaodong, Xia, Jiahong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876848/
https://www.ncbi.nlm.nih.gov/pubmed/27274239
http://dx.doi.org/10.2147/IJN.S103569
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author Shen, Qinglin
Peng, Caixia
Zhan, Yan
Fan, Liang
Wang, Mengyi
Zhou, Qing
Liu, Jue
Lv, Xiaojuan
Tang, Qiu
Li, Jun
Huang, Xiaodong
Xia, Jiahong
author_facet Shen, Qinglin
Peng, Caixia
Zhan, Yan
Fan, Liang
Wang, Mengyi
Zhou, Qing
Liu, Jue
Lv, Xiaojuan
Tang, Qiu
Li, Jun
Huang, Xiaodong
Xia, Jiahong
author_sort Shen, Qinglin
collection PubMed
description Selection of the optimal chemotherapy regimen for an individual cancer patient is challenging. The existing chemosensitivity tests are costly, time-consuming, and not amenable to wide utilization within a clinic. This limitation might be addressed by the recently proposed use of circulating tumor cells (CTCs), which provide an opportunity to noninvasively monitor response to therapy. Over the past few decades, various techniques were developed to capture and recover CTCs, but these techniques were often limited by a capture and recovery performance tradeoff between high viability and high efficiency. In this work, we used anti-epithelial cell adhesion molecule coated aptamer–poly (N-isopropylacrylamide) functionalized silicon nanowire substrates to capture and release epithelial cell adhesion molecule-positive CTCs at 32°C and 4°C, respectively. Then, we applied the nuclease to digest the aptamer to release the captured CTCs (near or at the end of the polymer brush), which cannot be released by heating/cooling process. High viability and purity CTCs could be achieved by decreasing the heating/cooling cycles and enzymatic treatment rounds. Furthermore, the time-saving process is helpful to maintain the morphology and enhance vitality of the recovered CTCs and is beneficial to the subsequent cell culture in vitro. We validated the feasibility of chemosensitivity testing based on the recovered HCC827 cells using an adenosine triphosphate–tumor chemosensitivity assay, and the results suggested that our method can determine which agent and what concentration have the best chemosensitivity for the culturing recovered CTCs. So, the novel method capable of a highly effective capture and recovery of high viability CTCs will pave the way for chemosensitivity testing.
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spelling pubmed-48768482016-06-07 Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing Shen, Qinglin Peng, Caixia Zhan, Yan Fan, Liang Wang, Mengyi Zhou, Qing Liu, Jue Lv, Xiaojuan Tang, Qiu Li, Jun Huang, Xiaodong Xia, Jiahong Int J Nanomedicine Original Research Selection of the optimal chemotherapy regimen for an individual cancer patient is challenging. The existing chemosensitivity tests are costly, time-consuming, and not amenable to wide utilization within a clinic. This limitation might be addressed by the recently proposed use of circulating tumor cells (CTCs), which provide an opportunity to noninvasively monitor response to therapy. Over the past few decades, various techniques were developed to capture and recover CTCs, but these techniques were often limited by a capture and recovery performance tradeoff between high viability and high efficiency. In this work, we used anti-epithelial cell adhesion molecule coated aptamer–poly (N-isopropylacrylamide) functionalized silicon nanowire substrates to capture and release epithelial cell adhesion molecule-positive CTCs at 32°C and 4°C, respectively. Then, we applied the nuclease to digest the aptamer to release the captured CTCs (near or at the end of the polymer brush), which cannot be released by heating/cooling process. High viability and purity CTCs could be achieved by decreasing the heating/cooling cycles and enzymatic treatment rounds. Furthermore, the time-saving process is helpful to maintain the morphology and enhance vitality of the recovered CTCs and is beneficial to the subsequent cell culture in vitro. We validated the feasibility of chemosensitivity testing based on the recovered HCC827 cells using an adenosine triphosphate–tumor chemosensitivity assay, and the results suggested that our method can determine which agent and what concentration have the best chemosensitivity for the culturing recovered CTCs. So, the novel method capable of a highly effective capture and recovery of high viability CTCs will pave the way for chemosensitivity testing. Dove Medical Press 2016-05-18 /pmc/articles/PMC4876848/ /pubmed/27274239 http://dx.doi.org/10.2147/IJN.S103569 Text en © 2016 Shen et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Shen, Qinglin
Peng, Caixia
Zhan, Yan
Fan, Liang
Wang, Mengyi
Zhou, Qing
Liu, Jue
Lv, Xiaojuan
Tang, Qiu
Li, Jun
Huang, Xiaodong
Xia, Jiahong
Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
title Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
title_full Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
title_fullStr Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
title_full_unstemmed Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
title_short Aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
title_sort aptamer–polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876848/
https://www.ncbi.nlm.nih.gov/pubmed/27274239
http://dx.doi.org/10.2147/IJN.S103569
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