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Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth

Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antim...

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Autores principales: Haque, Farazul, Alfatah, Md., Ganesan, K., Bhattacharyya, Mani Shankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876995/
https://www.ncbi.nlm.nih.gov/pubmed/27030404
http://dx.doi.org/10.1038/srep23575
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author Haque, Farazul
Alfatah, Md.
Ganesan, K.
Bhattacharyya, Mani Shankar
author_facet Haque, Farazul
Alfatah, Md.
Ganesan, K.
Bhattacharyya, Mani Shankar
author_sort Haque, Farazul
collection PubMed
description Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antimicrobial and anticancer properties. In this study, we investigated the effect of SL on C. albicans biofilm formation and preformed biofilms. SL was found to inhibit C. albicans biofilm formation as well as reduce the viability of preformed biofilms. Moreover, SL, when used along with amphotericin B (AmB) or fluconazole (FLZ), was found to act synergistically against biofilm formation and preformed biofilms. Effect of SL on C. albicans biofilm formation was further visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), which revealed absence of hyphae, typical biofilm architecture and alteration in the morphology of biofilm cells. We also found that SL downregulates the expression of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4, which possibly explains the inhibitory effect of SL on hyphae and biofilm formation.
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spelling pubmed-48769952016-06-06 Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth Haque, Farazul Alfatah, Md. Ganesan, K. Bhattacharyya, Mani Shankar Sci Rep Article Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antimicrobial and anticancer properties. In this study, we investigated the effect of SL on C. albicans biofilm formation and preformed biofilms. SL was found to inhibit C. albicans biofilm formation as well as reduce the viability of preformed biofilms. Moreover, SL, when used along with amphotericin B (AmB) or fluconazole (FLZ), was found to act synergistically against biofilm formation and preformed biofilms. Effect of SL on C. albicans biofilm formation was further visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), which revealed absence of hyphae, typical biofilm architecture and alteration in the morphology of biofilm cells. We also found that SL downregulates the expression of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4, which possibly explains the inhibitory effect of SL on hyphae and biofilm formation. Nature Publishing Group 2016-03-31 /pmc/articles/PMC4876995/ /pubmed/27030404 http://dx.doi.org/10.1038/srep23575 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Haque, Farazul
Alfatah, Md.
Ganesan, K.
Bhattacharyya, Mani Shankar
Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth
title Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth
title_full Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth
title_fullStr Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth
title_full_unstemmed Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth
title_short Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth
title_sort inhibitory effect of sophorolipid on candida albicans biofilm formation and hyphal growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876995/
https://www.ncbi.nlm.nih.gov/pubmed/27030404
http://dx.doi.org/10.1038/srep23575
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