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The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations

The zebra mussel Dreissena polymorpha is a well-established invasive model organism. Although extensively used in environmental sciences, virtually nothing is known of the molecular process of its shell calcification. By describing the microstructure, geochemistry and biochemistry/proteomics of the...

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Autores principales: Immel, Françoise, Broussard, Cédric, Catherinet, Bastien, Plasseraud, Laurent, Alcaraz, Gérard, Bundeleva, Irina, Marin, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877012/
https://www.ncbi.nlm.nih.gov/pubmed/27213644
http://dx.doi.org/10.1371/journal.pone.0154264
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author Immel, Françoise
Broussard, Cédric
Catherinet, Bastien
Plasseraud, Laurent
Alcaraz, Gérard
Bundeleva, Irina
Marin, Frédéric
author_facet Immel, Françoise
Broussard, Cédric
Catherinet, Bastien
Plasseraud, Laurent
Alcaraz, Gérard
Bundeleva, Irina
Marin, Frédéric
author_sort Immel, Françoise
collection PubMed
description The zebra mussel Dreissena polymorpha is a well-established invasive model organism. Although extensively used in environmental sciences, virtually nothing is known of the molecular process of its shell calcification. By describing the microstructure, geochemistry and biochemistry/proteomics of the shell, the present study aims at promoting this species as a model organism in biomineralization studies, in order to establish a bridge with ecotoxicology, while sketching evolutionary conclusions. The shell of D. polymorpha exhibits the classical crossed-lamellar/complex crossed lamellar combination found in several heterodont bivalves, in addition to an external thin layer, the characteristics of which differ from what was described in earlier publication. We show that the shell selectively concentrates some heavy metals, in particular uranium, which predisposes D. polymorpha to local bioremediation of this pollutant. We establish the biochemical signature of the shell matrix, demonstrating that it interacts with the in vitro precipitation of calcium carbonate and inhibits calcium carbonate crystal formation, but these two properties are not strongly expressed. This matrix, although overall weakly glycosylated, contains a set of putatively calcium-binding proteins and a set of acidic sulphated proteins. 2D-gels reveal more than fifty proteins, twenty of which we identify by MS-MS analysis. We tentatively link the shell protein profile of D. polymorpha and the peculiar recent evolution of this invasive species of Ponto-Caspian origin, which has spread all across Europe in the last three centuries.
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spelling pubmed-48770122016-06-09 The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations Immel, Françoise Broussard, Cédric Catherinet, Bastien Plasseraud, Laurent Alcaraz, Gérard Bundeleva, Irina Marin, Frédéric PLoS One Research Article The zebra mussel Dreissena polymorpha is a well-established invasive model organism. Although extensively used in environmental sciences, virtually nothing is known of the molecular process of its shell calcification. By describing the microstructure, geochemistry and biochemistry/proteomics of the shell, the present study aims at promoting this species as a model organism in biomineralization studies, in order to establish a bridge with ecotoxicology, while sketching evolutionary conclusions. The shell of D. polymorpha exhibits the classical crossed-lamellar/complex crossed lamellar combination found in several heterodont bivalves, in addition to an external thin layer, the characteristics of which differ from what was described in earlier publication. We show that the shell selectively concentrates some heavy metals, in particular uranium, which predisposes D. polymorpha to local bioremediation of this pollutant. We establish the biochemical signature of the shell matrix, demonstrating that it interacts with the in vitro precipitation of calcium carbonate and inhibits calcium carbonate crystal formation, but these two properties are not strongly expressed. This matrix, although overall weakly glycosylated, contains a set of putatively calcium-binding proteins and a set of acidic sulphated proteins. 2D-gels reveal more than fifty proteins, twenty of which we identify by MS-MS analysis. We tentatively link the shell protein profile of D. polymorpha and the peculiar recent evolution of this invasive species of Ponto-Caspian origin, which has spread all across Europe in the last three centuries. Public Library of Science 2016-05-23 /pmc/articles/PMC4877012/ /pubmed/27213644 http://dx.doi.org/10.1371/journal.pone.0154264 Text en © 2016 Immel et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Immel, Françoise
Broussard, Cédric
Catherinet, Bastien
Plasseraud, Laurent
Alcaraz, Gérard
Bundeleva, Irina
Marin, Frédéric
The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations
title The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations
title_full The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations
title_fullStr The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations
title_full_unstemmed The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations
title_short The Shell of the Invasive Bivalve Species Dreissena polymorpha: Biochemical, Elemental and Textural Investigations
title_sort shell of the invasive bivalve species dreissena polymorpha: biochemical, elemental and textural investigations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877012/
https://www.ncbi.nlm.nih.gov/pubmed/27213644
http://dx.doi.org/10.1371/journal.pone.0154264
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