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A dual protease approach for expression and affinity purification of recombinant proteins

We describe a new method for affinity purification of recombinant proteins using a dual protease protocol. Escherichia coli maltose binding protein (MBP) is employed as an N-terminal tag to increase the yield and solubility of its fusion partners. The MBP moiety is then removed by rhinovirus 3C prot...

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Detalles Bibliográficos
Autores principales:	Raran-Kurussi, Sreejith, Waugh, David S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877217/ https://www.ncbi.nlm.nih.gov/pubmed/27105777 http://dx.doi.org/10.1016/j.ab.2016.04.006

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