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Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI
Superparamagnetic iron oxide (SPIO) nanoparticles have been widely used in a variety of biomedical applications, especially as contrast agents for magnetic resonance imaging (MRI) and cell labeling. In this study, SPIO nanoparticles were stabilized with amphiphilic low molecular weight polyethylenim...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877342/ https://www.ncbi.nlm.nih.gov/pubmed/27216601 http://dx.doi.org/10.1186/s11671-016-1479-5 |
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author | Chen, Shuzhen Zhang, Jun Jiang, Shengwei Lin, Gan Luo, Bing Yao, Huan Lin, Yuchun He, Chengyong Liu, Gang Lin, Zhongning |
author_facet | Chen, Shuzhen Zhang, Jun Jiang, Shengwei Lin, Gan Luo, Bing Yao, Huan Lin, Yuchun He, Chengyong Liu, Gang Lin, Zhongning |
author_sort | Chen, Shuzhen |
collection | PubMed |
description | Superparamagnetic iron oxide (SPIO) nanoparticles have been widely used in a variety of biomedical applications, especially as contrast agents for magnetic resonance imaging (MRI) and cell labeling. In this study, SPIO nanoparticles were stabilized with amphiphilic low molecular weight polyethylenimine (PEI) in an aqueous phase to form monodispersed nanocomposites with a controlled clustering structure. The iron-based nanoclusters with a size of 115.3 ± 40.23 nm showed excellent performance on cellular uptake and cell labeling in different types of cells, moreover, which could be tracked by MRI with high sensitivity. The SPIO nanoclusters presented negligible cytotoxicity in various types of cells as detected using MTS, LDH, and flow cytometry assays. Significantly, we found that ferritin protein played an essential role in protecting stress from SPIO nanoclusters. Taken together, the self-assembly of SPIO nanoclusters with good magnetic properties provides a safe and efficient method for universal cell labeling with noninvasive MRI monitoring capability. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s11671-016-1479-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4877342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-48773422016-06-21 Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI Chen, Shuzhen Zhang, Jun Jiang, Shengwei Lin, Gan Luo, Bing Yao, Huan Lin, Yuchun He, Chengyong Liu, Gang Lin, Zhongning Nanoscale Res Lett Nano Express Superparamagnetic iron oxide (SPIO) nanoparticles have been widely used in a variety of biomedical applications, especially as contrast agents for magnetic resonance imaging (MRI) and cell labeling. In this study, SPIO nanoparticles were stabilized with amphiphilic low molecular weight polyethylenimine (PEI) in an aqueous phase to form monodispersed nanocomposites with a controlled clustering structure. The iron-based nanoclusters with a size of 115.3 ± 40.23 nm showed excellent performance on cellular uptake and cell labeling in different types of cells, moreover, which could be tracked by MRI with high sensitivity. The SPIO nanoclusters presented negligible cytotoxicity in various types of cells as detected using MTS, LDH, and flow cytometry assays. Significantly, we found that ferritin protein played an essential role in protecting stress from SPIO nanoclusters. Taken together, the self-assembly of SPIO nanoclusters with good magnetic properties provides a safe and efficient method for universal cell labeling with noninvasive MRI monitoring capability. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s11671-016-1479-5) contains supplementary material, which is available to authorized users. Springer US 2016-05-23 /pmc/articles/PMC4877342/ /pubmed/27216601 http://dx.doi.org/10.1186/s11671-016-1479-5 Text en © Chen et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Nano Express Chen, Shuzhen Zhang, Jun Jiang, Shengwei Lin, Gan Luo, Bing Yao, Huan Lin, Yuchun He, Chengyong Liu, Gang Lin, Zhongning Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI |
title | Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI |
title_full | Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI |
title_fullStr | Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI |
title_full_unstemmed | Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI |
title_short | Self-Assembled Superparamagnetic Iron Oxide Nanoclusters for Universal Cell Labeling and MRI |
title_sort | self-assembled superparamagnetic iron oxide nanoclusters for universal cell labeling and mri |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877342/ https://www.ncbi.nlm.nih.gov/pubmed/27216601 http://dx.doi.org/10.1186/s11671-016-1479-5 |
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