A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts

Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demon...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Helen, Yang, Yu, Xiang, Zhongmin, Yu, Lunyin, Chouitar, Jouhara, Yu, Jie, D'Amore, Natalie Roy, Li, Ping, Li, Zhi, Bowman, Douglas, Theisen, Matthew, Brownell, James E., Tirrell, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877488/
https://www.ncbi.nlm.nih.gov/pubmed/27247826
http://dx.doi.org/10.1155/2016/1274603
Descripción
Sumario:Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.

Ejemplares similares