A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts

Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demon...

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Autores principales: He, Helen, Yang, Yu, Xiang, Zhongmin, Yu, Lunyin, Chouitar, Jouhara, Yu, Jie, D'Amore, Natalie Roy, Li, Ping, Li, Zhi, Bowman, Douglas, Theisen, Matthew, Brownell, James E., Tirrell, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877488/
https://www.ncbi.nlm.nih.gov/pubmed/27247826
http://dx.doi.org/10.1155/2016/1274603
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author He, Helen
Yang, Yu
Xiang, Zhongmin
Yu, Lunyin
Chouitar, Jouhara
Yu, Jie
D'Amore, Natalie Roy
Li, Ping
Li, Zhi
Bowman, Douglas
Theisen, Matthew
Brownell, James E.
Tirrell, Stephen
author_facet He, Helen
Yang, Yu
Xiang, Zhongmin
Yu, Lunyin
Chouitar, Jouhara
Yu, Jie
D'Amore, Natalie Roy
Li, Ping
Li, Zhi
Bowman, Douglas
Theisen, Matthew
Brownell, James E.
Tirrell, Stephen
author_sort He, Helen
collection PubMed
description Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.
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spelling pubmed-48774882016-05-31 A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts He, Helen Yang, Yu Xiang, Zhongmin Yu, Lunyin Chouitar, Jouhara Yu, Jie D'Amore, Natalie Roy Li, Ping Li, Zhi Bowman, Douglas Theisen, Matthew Brownell, James E. Tirrell, Stephen J Biomark Research Article Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue. Hindawi Publishing Corporation 2016 2016-05-10 /pmc/articles/PMC4877488/ /pubmed/27247826 http://dx.doi.org/10.1155/2016/1274603 Text en Copyright © 2016 Helen He et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
He, Helen
Yang, Yu
Xiang, Zhongmin
Yu, Lunyin
Chouitar, Jouhara
Yu, Jie
D'Amore, Natalie Roy
Li, Ping
Li, Zhi
Bowman, Douglas
Theisen, Matthew
Brownell, James E.
Tirrell, Stephen
A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
title A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
title_full A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
title_fullStr A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
title_full_unstemmed A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
title_short A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
title_sort sensitive ihc method for monitoring autophagy-specific markers in human tumor xenografts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877488/
https://www.ncbi.nlm.nih.gov/pubmed/27247826
http://dx.doi.org/10.1155/2016/1274603
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