Hepatoprotective effect of Rosa canina fruit extract against carbon tetrachloride induced hepatotoxicity in rat

OBJECTIVE: The present study was conducted to investigate the hepatoprotective activity of hydro-ethanolic fruit extract of Rosa canina (R. canina) against carbon tetrachloride (CCl(4))-induced hepatotoxicity in rats. MATERIALS AND METHODS: Male Wistar albino rats were randomly divided into six groups of 8 animals of each, including control, toxic (CCl(4)), R. canina 250, 500, and 750 mg/kg + CCl(4) and R. canina 750 mg/kg alone. R. canina (p.o., daily) and CCl(4) (1 ml/kg twice a week, 50% v/v in olive oil, i.p.) were administered to animals for six weeks. Serum analysis was performed to assay the levels of aspartate aminotransferase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP), albumin (ALB), total protein (TP) and malondialdehyde (MDA). Biochemical observations were also supplemented with histopathological examination (haematoxylin and eosin staining) of liver section. RESULTS: Hepatotoxicity was evidenced by considerable increase in serum levels of AST, ALT, ALP, and lipid peroxidation (MDA) and decrease in levels of ALB and TP. Injection of CCL4 also induced congestion in central vein, and lymphocyte infiltration. Treatment with hydro-alcoholic fruit extract of R. canina at doses of 500 and 750 mg/kg significantly reduced CCl(4)-elevated levels of ALT, AST, ALP and MDA (p<0.01). The extract also increased the serum levels of ALB and TP compared to CCl(4) group (p<0.01) at the indicated dose Histopathological studies supported the biochemical finding. CONCLUSION: Our finding indicated hepatoprotective effects of the hydro-alcoholic fruit extract of R. canina on CCl(4)-induced hepatic damage in rats and suggested that these effects may be produced through reducing oxidative stress.