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Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus
Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric invers...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878303/ https://www.ncbi.nlm.nih.gov/pubmed/27223867 http://dx.doi.org/10.1590/0074-02760150470 |
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author | Cornel, Anthony J Brisco, Katherine K Tadei, Wanderli P Secundino, Nágila FC Rafael, Miriam S Galardo, Allan KR Medeiros, Jansen F Pessoa, Felipe AC Ríos-Velásquez, Claudia M Lee, Yoosook Pimenta, Paulo FP Lanzaro, Gregory C |
author_facet | Cornel, Anthony J Brisco, Katherine K Tadei, Wanderli P Secundino, Nágila FC Rafael, Miriam S Galardo, Allan KR Medeiros, Jansen F Pessoa, Felipe AC Ríos-Velásquez, Claudia M Lee, Yoosook Pimenta, Paulo FP Lanzaro, Gregory C |
author_sort | Cornel, Anthony J |
collection | PubMed |
description | Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3 (n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric subpopulations exist within the An. darlingi population at Manaus based on inversion frequencies. |
format | Online Article Text |
id | pubmed-4878303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-48783032016-05-25 Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus Cornel, Anthony J Brisco, Katherine K Tadei, Wanderli P Secundino, Nágila FC Rafael, Miriam S Galardo, Allan KR Medeiros, Jansen F Pessoa, Felipe AC Ríos-Velásquez, Claudia M Lee, Yoosook Pimenta, Paulo FP Lanzaro, Gregory C Mem Inst Oswaldo Cruz Articles Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3 (n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric subpopulations exist within the An. darlingi population at Manaus based on inversion frequencies. Instituto Oswaldo Cruz, Ministério da Saúde 2016-05 /pmc/articles/PMC4878303/ /pubmed/27223867 http://dx.doi.org/10.1590/0074-02760150470 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Cornel, Anthony J Brisco, Katherine K Tadei, Wanderli P Secundino, Nágila FC Rafael, Miriam S Galardo, Allan KR Medeiros, Jansen F Pessoa, Felipe AC Ríos-Velásquez, Claudia M Lee, Yoosook Pimenta, Paulo FP Lanzaro, Gregory C Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus |
title |
Anopheles darlingi polytene chromosomes: revised maps including
newly described inversions and evidence for population structure in
Manaus |
title_full |
Anopheles darlingi polytene chromosomes: revised maps including
newly described inversions and evidence for population structure in
Manaus |
title_fullStr |
Anopheles darlingi polytene chromosomes: revised maps including
newly described inversions and evidence for population structure in
Manaus |
title_full_unstemmed |
Anopheles darlingi polytene chromosomes: revised maps including
newly described inversions and evidence for population structure in
Manaus |
title_short |
Anopheles darlingi polytene chromosomes: revised maps including
newly described inversions and evidence for population structure in
Manaus |
title_sort | anopheles darlingi polytene chromosomes: revised maps including
newly described inversions and evidence for population structure in
manaus |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878303/ https://www.ncbi.nlm.nih.gov/pubmed/27223867 http://dx.doi.org/10.1590/0074-02760150470 |
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