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Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878305/ https://www.ncbi.nlm.nih.gov/pubmed/27223868 http://dx.doi.org/10.1590/0074-02760150412 |
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author | Verissimo-Villela, Erika Kitahara-Oliveira, Milene Yoko dos Reis, Ana Beatriz de Bragança Albano, Rodolpho Mattos Da-Cruz, Alda Maria Bello, Alexandre Ribeiro |
author_facet | Verissimo-Villela, Erika Kitahara-Oliveira, Milene Yoko dos Reis, Ana Beatriz de Bragança Albano, Rodolpho Mattos Da-Cruz, Alda Maria Bello, Alexandre Ribeiro |
author_sort | Verissimo-Villela, Erika |
collection | PubMed |
description | During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins. |
format | Online Article Text |
id | pubmed-4878305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-48783052016-05-25 Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents Verissimo-Villela, Erika Kitahara-Oliveira, Milene Yoko dos Reis, Ana Beatriz de Bragança Albano, Rodolpho Mattos Da-Cruz, Alda Maria Bello, Alexandre Ribeiro Mem Inst Oswaldo Cruz Short Communications During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins. Instituto Oswaldo Cruz, Ministério da Saúde 2016-05 /pmc/articles/PMC4878305/ /pubmed/27223868 http://dx.doi.org/10.1590/0074-02760150412 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communications Verissimo-Villela, Erika Kitahara-Oliveira, Milene Yoko dos Reis, Ana Beatriz de Bragança Albano, Rodolpho Mattos Da-Cruz, Alda Maria Bello, Alexandre Ribeiro Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents |
title | Functional complementation of Leishmania
(Leishmania) amazonensis AP endonuclease gene
(lamap) in Escherichia coli mutant strains
challenged with DNA damage agents |
title_full | Functional complementation of Leishmania
(Leishmania) amazonensis AP endonuclease gene
(lamap) in Escherichia coli mutant strains
challenged with DNA damage agents |
title_fullStr | Functional complementation of Leishmania
(Leishmania) amazonensis AP endonuclease gene
(lamap) in Escherichia coli mutant strains
challenged with DNA damage agents |
title_full_unstemmed | Functional complementation of Leishmania
(Leishmania) amazonensis AP endonuclease gene
(lamap) in Escherichia coli mutant strains
challenged with DNA damage agents |
title_short | Functional complementation of Leishmania
(Leishmania) amazonensis AP endonuclease gene
(lamap) in Escherichia coli mutant strains
challenged with DNA damage agents |
title_sort | functional complementation of leishmania
(leishmania) amazonensis ap endonuclease gene
(lamap) in escherichia coli mutant strains
challenged with dna damage agents |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878305/ https://www.ncbi.nlm.nih.gov/pubmed/27223868 http://dx.doi.org/10.1590/0074-02760150412 |
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