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A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline

Imaging of RNAs and proteins in specific tissues has opened ample avenues to understand gene expression during development. Recently, a fluorescent in situ RNA hybridization (FISH) method has been developed to analyze the spatio-temporal expression patterns of endogenous mRNAs. However, combining FI...

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Detalles Bibliográficos
Autores principales: Yoon, Dong Suk, Pendergrass, DeQwon L., Lee, Myon-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878318/
https://www.ncbi.nlm.nih.gov/pubmed/27257608
http://dx.doi.org/10.1016/j.mex.2016.05.001