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A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline
Imaging of RNAs and proteins in specific tissues has opened ample avenues to understand gene expression during development. Recently, a fluorescent in situ RNA hybridization (FISH) method has been developed to analyze the spatio-temporal expression patterns of endogenous mRNAs. However, combining FI...
Autores principales: | Yoon, Dong Suk, Pendergrass, DeQwon L., Lee, Myon-Hee |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878318/ https://www.ncbi.nlm.nih.gov/pubmed/27257608 http://dx.doi.org/10.1016/j.mex.2016.05.001 |
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